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allied
academies
September 10-11, 2018 | Paris, France
&
Joint Event
Otolaryngology: ENT Surgery
6
th
International Conference on
Cell & Stem Cell Research
World Congress and Expo on
Journal of Otolaryngology Online Journal | Volume 8
Different aspect of electro taxis in long term culture cell related to senescence
Seung Hee Hong, Mi Hee Lee, Min-Ah Koo, Gyeung Mi Seon
and
Jong-Chul Park
Yonsei University College of Medicine, Republic of Korea
S
tem cells may differentiate into various cell lineages. Thus,
these cells are promising tools for tissue engineering and cell
therapies. But stem cells have limited lifespan in vitro, so the
use of stem cells are restricted. It has been reported that long-
term culture of stem cells causes the cells undergo senescence
with cell morphology, alteration in differential potential and
proliferating ability. In this study, we confirmed the possibility
of applying direct current electric field to cells, known as electro
taxis, to be a useful way to distinct cell. We used 3 types of
stem cells (TMSC, hMSC, ADSC) and 3 passages (P5, P10, P15)
for each stem cells in every experiment. We performed MTT
assay to evaluate proliferative ability of 3 types of stem cells.
The proliferation rate of stem cells cultured for long term
was significantly decreased compared with the early phase
passage 5. One of well-known senescence marker, senescence-
associated ß-galactosidase was stained, and its activity was
detected. The number of ß-galactosidase stained cells were
increased in long term culture compared with the early phase.
The activity of ß-galactosidase shown similar pattern compared
with ß-galactosidase staining. In electric induced cell migration,
we applied cells in charge of 1000µA for 3 hours in customized
agar-salt electro taxis incubator and chamber system.
Early cultured cells move toward anode with directedness
direction. But in long-term culture cells directedness has
been decreased. In conclusion, our result showed that long
term culture of stem cells undergoes senescence which can
be known by proliferation assay and senescence-associated
ß-galactosidase assay. Also, in electro taxis result, different
result about passage increasing could be influenced by stem
cell senescence which is similar aspect shown in senescence
assay. Therefore, applying direct current electric field to
cells can be another method to confirm senescence cells.
Speaker Biography
Seung Hee Hong is currently pursuing in the Yonsei University College of Medicine at
Republic of Korea.
e:
parkjc@yuhs.acENT and Stem Cell 2018, Volume 8
DOI: 10.4066/2250-0359-C1-003