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allied
academies
September 10-11, 2018 | Paris, France
&
Joint Event
Otolaryngology: ENT Surgery
6
th
International Conference on
Cell & Stem Cell Research
World Congress and Expo on
Journal of Otolaryngology Online Journal | Volume 8
ROS-induced cell sheet delivery method based on photofunctional polymer film
Min-Ah Koo, Mi Hee Lee, Gyeung Mi Seon, Seung Hee Hong
and
Jong-Chul Park
Yonsei University College of Medicine, Republic of Korea
C
ell transplantation is one of the promising technologies for
the repair and regeneration of damaged tissues. Previous
approaches have been applied the direct injections of cell
suspensions and the implantation of biodegradable three-
dimensional scaffolds seeded cells. However, these treatments
have limits due to poor localization of the injected cells and
insufficient delivery of oxygen and nutrients to cells, host
inflammatory reactions by scaffolds. Cell sheet engineering was
recently proposed as a new approach transplanting cell sheets
to the lesion without scaffolds. To harvest cultured cell sheets,
most studies has focused on culture surface property variations
(e.g. wettability, pH, electricity, magnetism). Photodynamic
action causes production of reactive oxygen species (ROS) by
interaction of light with photosensitive agents and oxygen.
This study investigated ROS-induced cell detachment system
to transfer cell sheet directly at target area without harvesting
procedure. To produce exogenous ROS, a photosensitizer-
immobilizedpolymer filmwas preparedby spin coatingmethod.
Treatment of green light to activate photosensitizer contained
in the film generates ROS, which in turn causes cell sheet
detachment from the film. We have found optimum conditions
to detach an intact cell sheet with extracellular matrix (ECM)
protein. This method is capable of efficiently transplanting
cells in a simple process, by contacting the cultured film with
the lesion where the cell is to be delivered and irradiating light.
Also, the study demonstrated in vivo efficacy of applying this
approach to subcutaneous tissues of nude mice. Therefore,
the study indicates that ROS-induced method for cell sheet
transplantation has potential application in tissue engineering.
Speaker Biography
Min-Ah Koo is currently pursuing in the Yonsei University College of Medicine at Republic
of Korea.
e:
parkjc@yuhs.acENT and Stem Cell 2018, Volume 8
DOI: 10.4066/2250-0359-C1-003