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Journal of Microbiology: Current Research | Volume 2
November 01-02, 2018 | London, UK
7th EuropeanClinical Microbiology Congress
4th International Conference onOphthalmology and Eye Disorder
Joint Event
&
Prevalence of enterovirus serotypes in children with encephalitis/meningitis in Shanghai, China,
2016 ~ 2017
Jingjing Li
Children’s Hospital of Fudan University, China
Background: Eneterovirus (EV) is a major cause of viral
encephalitis/meningitis. This study aimed to investigate the
prevalence of enterovirus-associated encephalitis/meningitis
and the distribution of enterovirus serotypes in children with
encephalitis/meningitis in Shanghai during 2016~2017.
Methods: We collected cerebrospinal fluid specimens from
pediatric patients with encephalitis/meningitis and stool
specimens from children with viral encephalitis/meningitis
followed with hand-foot-mouth disease (HFMD) during 2016
~2017. The nested RT-PCR and sequencing were performed to
identify the enteroviruses and serotypes.
Results: During 2016 ~2017, we obtained 295 non-duplicated
cerebrospinal fluid specimens from children with clinically
diagnosis viral encephalitis/meningitis, and enterovirus was
positive in 163 (55.25%) specimens. Of which, 139 and 156
specimens were taken from inpatients and outpatients,
respectively. Enterovirus was positive in 66 (47.48%) and
97 (62.18%) cerebrospinal fluid specimens from inpatients
and outpatients, respectively. Among inpatients with viral
encephalitis/meningitis, 11 serotypes were identified including
Echovirus 30 (E30, 42.42%), Coxsackievirus A6 (CV-A6, 12.12%),
CV-A5 (10.61%), E6 (9.09%), E11 (7.58%), CV-A2 (4.55%), E9
(4.55%), CV-B5 (4.55%), CV-A10 (1.52%), CV-B3 (1.52%), E14
(1.52%). Among outpatients with viral encephalitis/meningitis,
13 serotypes were identified, including CV-A6 (31.96%), E30
(23.71%), CV-A10 (14.43%), E6 (7.22%), E9 (5.15%), CV-A2
(4.12%), CV-A9 (4.12%), CV-A5 (3.09%), CV-B5 (2.06%), EV-
A71 (2.06%), E14 (1.03%), CV-B4 (1.03%). Of the 5 cases with
critically severe encephalitis who all survived, E9, CV-A2 and E6
was identified in 2 cases and 1 case, respectively. Among 163
EV-associated encephalitis/meningitis cases, children aged >4
years old accounted for the most cases with 79.14% (129/163).
Besides, we obtained 61 stool specimens from children with
viral encephalitis/meningitis followed with HFMD. And EV was
positive in 56 (91.80%) specimens. 11 serotypes were identified
including EV-A71 (85.71%), CV-A2 (5.36%), CV-A16 (23.57%),
CV-A6 (3.57%), CV-A5 (1.79%). All encephalitis/meningitis
followed with HFMD were mild cases. Of which, children aged
<4 years old accounted for the most cases with 73.22% (41/56).
Conclusion: Multiple enterovirus serotypes co-circulated
among children in Shanghai. Non-EV-A71 enteroviruses were
responsible for viral encephalitis/meningitis and E30 and CV-A6
were frequent serotype responsible for encephalitis/meningitis.
And school children were more susceptible to EV-associated
encephalitis/meningitis than preschool child.
e:
Notes:
Clinical Microbiology and Eye 2018, Volume 2
DOI: 10.4066/2591-8036-C1-003