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allied
academies
Journal of Microbiology: Current Research | Volume 2
November 01-02, 2018 | London, UK
7
th
European
Clinical Microbiology Congress
4
th
International Conference on
Ophthalmology and Eye Disorder
Joint Event
&
Salmonella
Typhimurium and
Salmonella
Enteritidis infections in sporadic diarrhea in children: Source
tracing and resistance to third-generation cephalosporins and ciprofloxacin
Zhougqiu Wei
Children’s Hospital of Fudan University, China
Objectives:
This study is aimed to trace the transmission source
of
Salmonella
Typhimurium and
Salmonella
Enteritidis strains
associatedwith enteric infections in Shanghainese children, and
understand the molecular mechanism of resistance to third-
generation cephalosporins and ciprofloxacin.
Methods:
The profiles of pulsed-field gel electrophoresis (PFGE)
were compared among the isolates from children, animal and
environment. Antimicrobial susceptibility was determined
using the minimal inhibitory concentrations and Kirby-Bauer
disk diffusion method. Genes mediating extended-spectrum
β-lactamase (ESBL) producing were identified using PCR and
sequencing.
Results:
Based on PFGE patterns, 49 (33.1%) of 148 human
Salmonella
Typhimurium isolates in the dominant PFGE
clusters were genetically related to the isolates from poultry
source, environment water, aquatic products and reptiles and
97(97.0%) of 100 human
Salmonella
Enteritidis isolates were
genetically related to isolates from poultry and water. The
rates of resistance to ceftriaxone among clinical
Salmonella
Typhimurium and
Salmonella
Enteritidis isolates were 42.0%
and 14.2%, respectively. Besides, 35.1% of clinical
Salmonella
Typhimurium isolates were resistant to ciprofloxacin. The rates
of resistance to cefotaxime and ciprofloxacin among
Salmonella
Typhimurium isolates from freshwater food animals and
Salmonella
Enteritidis isolates from pork meat were 13% and
69.6%, and 18.2%and 9.1%, respectively. Of the 64 ESBL/AmpC-
producing strains, CTX-M, TEM, DHA and CMY were found in
86.0%, 62.5%, 7.8%, 3.1% and 3.1% of isolates, respectively.
Conclusions:
The transmission sources of
Salmonella
Typhimurium and
Salmonella
Enteritidis infections in
Shanghainese children were diverse. The high prevalence of
resistance to third-generation cephalosporins and ciprofloxacin
mediated by multiple molecular mechanisms need continuous
attention and intervention.
e:
zqwei0563@163.comClinical Microbiology and Eye 2018, Volume 2
DOI: 10.4066/2591-8036-C1-003