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Journal of Microbiology: Current Research | Volume 2

November 01-02, 2018 | London, UK

European

Clinical Microbiology Congress

International Conference on

Ophthalmology and Eye Disorder

Joint Event

The

in-vitro

anti-methicillin resistant

Staphylococcus aureus

(MRSA) activity of

Garcinia binucao

(Choisy)

Alyanna Maachah P Hipe

University of Santo Tomas, Philippines

he use of antibiotics has paved the way for the health

improvement of the people, but its use and misuse have

also caused several problems which has alarmed the clinical

community. Natural products, specifically underutilized and

indigenous fruits of the Philippines may have promising effects

compared to chemically synthesized antibiotics.

Garcinia

binucao

, an evergreen fruit tree, is abundant in the Visayas

region and its only known function is as a souring agent

among the locals. This study shall identify Batuan’s bioactive

compounds and screen the fruit and leaf extract against

methicillin-resistant

Staphylococcus aureus

(MRSA), a clinically

significant multi-drug resistant pathogen. The fruits and leaves

will be procured in Jaro, Iloilo and will be extracted using

ethanol and methanol. These crude extracts will be prepared

for an antimicrobial susceptibility assay and a time-kill assay.

Transmission electron microscopy will be done to observe

cell ultrastructure on the extract’s exposure to MRSA which,

may shed light on the extract’s possible mechanism of action.

Clinical Microbiology and Eye 2018, Volume 2

DOI: 10.4066/2591-8036-C1-003