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allied
academies
Journal of Microbiology: Current Research | Volume 2
November 01-02, 2018 | London, UK
7
th
European
Clinical Microbiology Congress
4
th
International Conference on
Ophthalmology and Eye Disorder
Joint Event
&
Screening, identification and antimicrobial activity of mycoparasitic fungus (
Aspergillus
sp.) from Phil-
ippine aglibut sweet tamarind
Crisanto A Miclat
Saint Joseph Thare School, Philippines
D
ue to the increasing resistance of pathogenic
microorganisms,
Aspergillus
sp. isolated from aglibut
sweet tamarind’s bark of Pampanga State Agricultural
University was evaluated for potential mycoparasitism and
antimicrobial activity. The micrograph obtained from Scanning
Electron Microscopy (SEM) analysis reveals that
Aspergillus
sp. is a potential mycoparasite; further, its identity was 99%
which was confirmed through 18s rDNA of its ITS1 forward
and ITS4 reverse sequences by Polymerase Chain Reaction
(PCR) amplification and sequencing. Moreover, Thin-layer
Chromatography (TLC) was used to identify the bioactive
compounds of
Aspergillus
sp. The chemical groups such as
glycosidic flavonoid, alkaloid and anthrones were also present
which can express the desired activity. Complete Randomized
Design (CRD) was carried out with the following treatments;
T1 (suspensions) - control (DMSO) and + control (streptomycin
for bacteria: Ketoconazole for fungus). Paper-disc diffusion
confirms that the suspensions of
Aspergillus
sp. have significant
antimicrobial potential as shown in the zones of inhibition in
S.
aureus
and
S. cerevisiae
but with lower activity in
E. coli
. Thus,
Aspergillus
sp. is a potential mycoparasite and source of new
drugs and drug products.
e:
miclatcrisantoaquinojr@yahoo.comClinical Microbiology and Eye 2018, Volume 2
DOI: 10.4066/2591-8036-C1-003