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Journal of Microbiology: Current Research | Volume 2

November 01-02, 2018 | London, UK

European

Clinical Microbiology Congress

International Conference on

Ophthalmology and Eye Disorder

Joint Event

Human RAD9B variant 2 regulates the phosphorylation of CHK2 kinase

Yogendra Kumar Verma

Bangor University, United Kingdom

uman RAD9A and RAD9B protein are two isoforms of the

DNA lesion response proteinRAD9. RAD9AandRad9B form

the Rad9-Rad1-Hus1 checkpoint ring at sites of DNA damage.

TheRAD9proteinparticipates in theDNArepair process through

regulating cell cycle checkpoints and the apoptotic pathway. The

checkpoint kinases CHK1 and CHK2 are associated with single

and double stranded DNA damage jointly with ATR and ATM

kinase, respectively. RAD9A and RAD9B isoforms differ from

each other by their amino acid sequence; RAD9B is larger than

RAD9A. So far it is unknown why human cells express to related

Rad9 proteins. The RAD9B gene encodes four protein splice

variants and the results presented here identify splice variant

2 as a regulator or CHK2 phosphorylation. Only over-expression

of variant 2 but not variant 1 in HEK293 cells results in the

hyper-phosphorylation of CHK2 in undamaged cells. It is still not

yet clear which kinase is involved in the aberrant modification

of CHK2. This is the first evidence identifying a specific function

for Rad9B and more specifically for one of its protein variants.

Clinical Microbiology and Eye 2018, Volume 2

DOI: 10.4066/2591-8036-C1-003