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N o v e m b e r 1 2 - 1 3 , 2 0 1 8 | R o m e , I t a l y
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
&
CHROMATOGRAPHY AND SEPARATION SCIENCE
World Congress on
SATELLITE AND SPACE MISSIONS
International Conference and Exhibition on
Chromatography 2018 & Satellite 2018 Journal of Chemical Technology and Applications| Volume 2
Anupam Maurya et al., J Chem Tech App 2018, Volume 2
LIQUID-LIQUID PARTITION
CHROMATOGRAPH: AN EFFICIENT SOLID
SUPPORT LESS CHROMATOGRAPHIC
TECHNIQUE FOR THE SEPARATION OF
BIOACTIVE PHYTOMOLECULES
Anupam Maurya, Subash Chandra Verma and Ravinder Singh
Ministry of AYUSH, India
Phytomolecules are secondary metabolites of plants origin which have
various biological activities and became key source for the discovery of
new drugs. Last 20 years, almost 50% drugs directly or indirectly derived from
natural products for Human welfare. All phytomolecules, often exit as a very
complex mixture from which the product of interest must be isolated and pu-
rified, separation of these molecules from the plants is very tedious and time
taking. At present, the used adsorbents SiO2 and Al2O3 are not chemical in-
ert. Separation of natural products on alumina or silica gel sometimes results
in recovery of only 70-90%. Sometimes severe losses of valuable materials
result because of irreversible adsorption on a solid support. In addition, isola-
tion of artifacts has also been reported due to chemical reaction of the sub-
strates with solid phase adsorbents. Introduction of Liquid- Liquid Partition
Chromatography (CCC, CPC etc.) provided new dimension in area of sepa-
ration, isolation and purification of phytomolecules, in which both stationary
and mobile phase are liquid. The separation of Compounds in this technique
is based on their Partition Coefficient (K), in which the compounds distribute
between biphasic solvent systems. Techniques having number of advantages
when it compared with the more traditional liquid-solid separations method:
(i) It can be used in both normal and reverse phase; (ii) It is less time taking
& economic; (iii) No irreversible adsorption (iv) Recovery more than 90%.; (v)
Tailing minimized; (vi) Low risk of sample denaturation; (vii) Low solvent con-
sumption & High loading capacity. With these above said advantages, tech-
niques are gaining popularity as modern separation technique.
We have also successfully applied Fast Centrifugal Partition Chromatogra-
phy (FCPC) for the separation and isolation of various phytomolecules like
iridoids, nitrile glycosides, triterpenoids, alkaloids and steroidal glycosides
& glycoalkaloids. The detail procedures of this method will be disused in
WCCSS 2018.
Anupam Maurya has completed his PhD at the age of
31 from CSIR-CIMAP India. Currently, he is working as a
Scientist (Scientific Officer) at Pharmacopoeia Commis-
sion for Indian Medicine and Homoeopathy, India. He has
published more than 24 papers in reputed journals and
granted one US patent. His current citation of publication
is 238 and H-index is 10. He has carried substantial work
on the isolation of Phytomolecules by Partition Chro-
matographic Technique and development of analytical
methods for Indian Medicinal Plants.
BIOGRAPHY