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N o v e m b e r 1 2 - 1 3 , 2 0 1 8 | R o m e , I t a l y
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
&
CHROMATOGRAPHY AND SEPARATION SCIENCE
World Congress on
SATELLITE AND SPACE MISSIONS
International Conference and Exhibition on
Chromatography 2018 & Satellite 2018 Journal of Chemical Technology and Applications| Volume 2
Areeg Z Alkarali et al., J Chem Tech App 2018, Volume 2
DEVELOPMENT OF HPLC METHOD FOR
SIMULTANEOUS DETERMINATION OF
FOUR STEROID HORMONES IN DIFFERENT
MATRICES
Areeg Z Alkarali, Lubna Kormod, Randa A Abdelsalam
Ghada M Hadad and Ahmed E El-Gendy
Misr International University, Egypt
Since animal products are a major source of human exposure to steroids.
The extensive use of these hormones resulted in a critical request for
accurate, sensitive, simple and applicable methods for the determination of
these steroid hormones in pure form and in different matrices as hen eggs,
chicken liver and Tilapia farming pond water. The aim of this work is to devel-
op a reliable liquid chromatography coupled with mass spectrometric method
for the determination of selected steroid hormones in complicated matrices
as hen egg, chicken liver and Tilapia farming pond water. Using solid phase
and liquid liquid extraction as methods of sample preparation. In the pres-
ent study LCMS/MS method was demonstrated for the simultaneous sep-
aration and quantification of 4 steroid hormones Ethinlyestradiol, 17 alpha
Methyl testosterone, testosterone, and progesterone. Using mobile phase of
methanol and 0.1% Formic in ratio (70:30) at different m/z ratios. The meth-
od validation was carried out on each hormone showing: linearity for Ethinyl-
estradiol 0.5 µg/ml to 30 µg/ml r2=0.9997, 17α-methyl testosterone 0.5 µg/
ml – 20 µg/ml r2=0.9999, Testosterone 0.5 µg/ml - 20 µg/ml r2=0.9999 and
progesterone 0.5 µg/ml- 20 µg/ml r2=0.9999. LOD and LOQ of (0.7 and 2.12),
(0.23 and 0.69),(0.35 and 1.08). and (0.36 and 1.11) for Ethinylestradiol, 17 α
methyl testosterone, testosterone and progesterone respectively. The meth-
od was validated using the ICH guidelines and successfully applied on egg,
chicken liver and Tilapia pond freshwater samples from Egypt.
Areeg Z Alkarali has completed her bachelor’s degree in
pharmaceutical sciences in 2012 fromMisr International
University; after that she finished her master’s degree in
pharmaceutical analytical chemistry in 2018 from Suez
Canal University.
BIOGRAPHY