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N o v e m b e r 1 2 - 1 3 , 2 0 1 8 | R o m e , I t a l y
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
&
CHROMATOGRAPHY AND SEPARATION SCIENCE
World Congress on
SATELLITE AND SPACE MISSIONS
International Conference and Exhibition on
Chromatography 2018 & Satellite 2018 Journal of Chemical Technology and Applications| Volume 2
Silvestro L et al., J Chem Tech App 2018, Volume 2
APPLICATIONS OF ICP-MS HYPHENATED
TO HPLC ON BIOLOGICAL SAMPLES FOR PK
STUDIES
Silvestro L, Maurer M, Ochinzi E and Rizea Savu S
3S-Pharmacol. Cons. & Res. GmbH, Germany
ICP-MS is a well-established analytical technique mainly applied to metal
determinations both in inorganic chemistry and biological samples (envi-
ronmental and toxicological studies). The possibility of ICP-MS hyphenation
to separation techniques, like HPLC and CZE, has shown interesting results
in particular for the speciation of inorganic ions while applications in biolog-
ical samples for pharmacological studies are rare. Along the years several
pharmaceutical products containing metal, like iron, in high molecular weight
complexes have been developed presenting difficult analytical problems es-
pecially in biological samples; indeed the determination of the metal itself is
of modest values without concomitant information like molecular weight and
polarity. In this context the combination with HPLC, permitting separations
based on molecular weight, ionic strength and hydrophobicity, is an ideal tool
to develop selectivemethods for such complex compounds. The possibility to
correctly quantitate an element, irrespective of the chemical structure where
is contained, is another interesting feature of ICP-MS and it offers interesting
possibilities of metabolites quantitation and identification in biological sam-
ples of drugs containing not just metals but also sulfur and/or phosphor. A
series of applications centered on these two classes of compounds will be
presented showing the great potential of ICP-MS as a detector in combination
with HPLC; as always nothing is perfect and therefore the limitations of this
analytical approach will be discussed, in particular matrix effects and mobile
phase compatibility.
Silvestro L graduated in Medicine in Turin (Italy) in 1984
and specialized in Pharmacology in 1988. From 1989
he is applying HPLC-MS in quantitation of bioanalytical
samples as well as identification of drug metabolites. In
1990 he began to study the application of ICP-MS and
other elemental analysis methods, hyphenated to HPLC,
on bioanalytical samples. In 1996 he has co-founded
3S-Pharmacological Consultation & Research GmbH, a
consultation company and CRO, in Germany and is still
actively involved in the development of innovative ana-
lytical methods. From 1998 the company has expanded
the activity in East Europe (Romania, Moldavia) creating
an analytical laboratory in Romania (Bucharest). In his
scientific activity he has contributed to more then 60 arti-
cles in international scientific journals.
BIOGRAPHY