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Journal of Biotechnology and Phytochemistry
Volume 1 Issue 3
Chemistry World 2017
Page 61
November 13-15, 2017 Athens, Greece
7
th
World Congress on
Chemistry
MIP nanoparticles in diagnostics and
bioimaging
Sergey A. Piletsky
University of Leicester, United Kingdom
Two years ago we have made a major breakthrough in MIP
technology developing solid-phase approach for preparation
of soluble molecularly imprinted nanoparticles (nanoMIPs)
with exquisite affinity and selectivity for their templates1.
The success came from combining controlled radical
polymerisation2 with an affinity separation step performed
on surface-immobilised template3. This approach represents
the state-of-the-art in nanoMIP synthesis: not only are soluble
particles with defined size (20-200 nm) and a narrow size
distribution produced in one hour, they possess subnanomolar
dissociation constants for their respective targets, they can
be easily functionalised with fluorescent, electrochemical or
magnetic labels, and the immobilised template can be re-
used. High affinity nanoMIPs were made for small molecules,
proteins, membrane proteins and virus particles4.
The main practical niches for application of synthesised
nanoMIPs are diagnostics, imaging and drug delivery.
Particularly exciting is an opportunity to use MIP sensors
in companion diagnostics. Members of our team have used
nanoMIPs successfully as a replacement for antibodies in
ELISA-type assays, electrochemical and optical sensors5.
The exciting examples of our work with in vivo application
potential are targeting membrane receptors, enzymes and
quorum sensing molecules6. Very encouraging facts that
enables practical applications of MIPs in vivo are lack of
polymer toxicity, ability of nanoMIPs to penetrate into cells
and to pass blood barrier. Current paper discusses challenges
and opportunities that are faced by MIP technology in the
light of these developments.
sp523@le.ac.ukJ Biotech and Phyto 2017