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Journal of Biotechnology and Phytochemistry
Volume 1 Issue 3
Chemistry World 2017
Notes:
Page 57
November 13-15, 2017 Athens, Greece
7
th
World Congress on
Chemistry
Determination of Some Antidiabetics by High
Performance Liquid Chromatography
Sena Caglar Andac, Nusret KARA, Burcu Elif Terlemez
and
Seda Kahramaner
Istanbul University, Faculty of Pharmacy, Department of Analytical Chemistry,
TURKEY
D
iabetes is a disorder of the metabolism mostly seen as
a combination of inherited or environmental factors
and resulted with over increase of blood glucose level
(hyperglycemia), the prevalence is increasing day by day in
Turkey and in the world. Dipeptidyl peptidase-4 inhibitors (DPP-
4s), gliptins, are a new class of drugs for oral hypoglycemic and
use for the treatment of type 2 Diabetes. Sitagliptin, vildagliptin
and saxagliptin are the members of the gliptin drugs which are
available in the market in Turkey. The advantages of gliptin drugs
are differ from oral hypoglycemic drugs used in the treatment
of type 2 diabetes like sulphonylureas, biguanids, α-glucosidase
inhibitors and meglinids by oral implementation due to its non-
peptide structure, and less side effects to the gastrointestinal
system since the incretin receptors are not affected directly. In
this study a fast and simultaneous HPLC method was developed
for the determination of sitagliptin, vildagliptin and saxagliptin
in tablets. A Thermo Ultimate 3000 HPLC was used for the
chromatographic method development. Separation was achieved
on a Gemini C18 (4.6x250mm, 5µ) HPLC column with a mobile
phase combination of methanol: ortho phosphoric acid, in
gradient elution. Analytes were detected both on 225 and 212
nm wavelengths. The developed method was validated and will
be applied to biological samples. This work was carried out at
Istanbul University, Faculty of Pharmacy as an undergraduate
research project.
Biography
Sena CaglarAndac has completed her PhD from Istanbul University and Postdoctoral
studies from Medical Center of Munich University, Laboratory of Bio-Separation.
Currently, she is working as an Associate Professor at Istanbul University, Faculty
of Pharmacy. She has researches published in liquid chromatography mass
spectrometry for biological fluid analysis of drugs, on-line solid phase extraction
coupled liquid chromatography, determination of drugs and degradation studies by
high performance liquid chromatography, spectrophotometry and spectrofluorimetry.
sena@istanbul.edu.trSena Caglar Andac et al., J Biotech and Phyto 2017