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May 13-14, 2019 | Prague, Czech Republic
Chemistry and Medicinal Chemistry
9th World Congress on
Page 57
Asian Journal of Biomedical and Pharmaceutical Sciences | Volume 9 ISSN: 2249-622X
Yuh-Lin Wang
National Taiwan University, Taiwan
Rapid bacterial antibiotic susceptibility tests based on Simple Surface-
Enhanced-Raman Spectroscopic Biomarkers
Rapid bacterial antibiotic susceptibility tests (AST)
are important to help reduce the mortality of sepsis
patients, the widespread misuse of antibiotics and the
growing drug-resistance problem. We discovered that,
when a susceptible strain of bacteria is exposed to an
antibiotic, the intensity of specific biomarkers in its surface
enhanced Raman scattering (SERS) spectra drops evidently
in two hours. The discovery has been exploited for rapid
antibiotic susceptibility test (AST) – dubbed SERS-AST, of
bacteria cultured from the blood samples of sepsis patients.
By applying 3 ~ 4 antibiotics to every clinical sample, a total
of more than six hundred cases of SERS-AST was conducted;
and the overall successful rate of identifying the bacteria’s
antibiotic susceptibility was 95.7%.
The biomolecules responsible for these bacterial SERS
biomarkers have been identified as several purine
derivative metabolites involved in bacterial purine salvage
pathways. Using ultra-performance liquid chromatography/
electrospray ionization-mass spectrometry (UPLC/ESI-MS),
the time dependences of the concentrations of these
molecules were measured. Surprisingly, a single S. aureus
and E. coli cell were found to release millions of adenine
and hypoxanthine into a water environment in an hour
respectively. The implications of these findings about the
molecular origin of the SERS biomarkers to the emergent
SERS-AST method will be addressed in conjunction with
other emergent AST methods.
Speaker Biography
Yuh-Lin Wang, Dr. Physics, specializes in the creation and applications
of novel nanostructures that are formed solid surfaces via constrained
self-organization processes. He is a co-author of more than 150 papers
and the recipient of several awards including the Prime Minister Award
for Outstanding Contribution in Science and Technology, Taiwan and
Academic Award, Ministry of Education, Taiwan. He is a fellow of
American Physical Society, U.S.A. He is an adjunct professor of the
department of physics, National Taiwan University, Taiwan.
e:
Yuh-Lin Wang, Asian J Biomed Pharmaceut Sci, Volume:9
DOI: 10.4066/2249-622X-C2-019
Notes: