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May 13-14, 2019 | Prague, Czech Republic
Chemistry and Medicinal Chemistry
9th World Congress on
Page 50
Asian Journal of Biomedical and Pharmaceutical Sciences | Volume 9 ISSN: 2249-622X
Yaw-Kuen Li
NCTU, Taiwan
Strategy for constructing antifouling biosensors
Biosensors commonly suffer from seriously non-specific
protein adsorption while measuring real samples
in human serum and consequently lead to unreliable
responses. To improve the accuracy of detection, biosensor
modified with an antifouling surface is indispensable.
Though many strategies for reducing non-specific
interaction have been developed, the task has not been
solved with an acceptable outcome. In particular, after
installing the biorecognizers, such as antibodies, for specific
targets, the anti-fouling feature of a biosensor is commonly
destroyed. How to overcome this problem becomes a
main issue of biosensing. We propose and demonstrate
an effective protocol via electrodeposition of charged-
aniline derivatives for the fabrication of biosensors with
promising antifouling ability. The antifouling layer, denoted
as (N+S) layer, was constructed on various electrodes via
electrodeposition by using the mixture (1:1 molar ratio) of
two opposite charged-aniline derivatives, 4-amino-N, N,
N-trimethylanilinium (N) and 4-aminobenzenesulfonate
(S). In order to know the power of antifouling of (N+S)
surface, we compared its cyclic voltammetric property
and the feature of human serum adhesion with the
common antifouling surfaces (zwitterionic layers and poly
ethylene glycol layers). All the tested surfaces gave similar
antifouling power. Yet, the (N+S)-modified surface showed
excellent conductivity. The antifouling efficiency of the
(N+S) surface is greatly improved with the addition of 1 %
sarcosine in human serum as compared with other tested
surfaces. On top of the “charged layer”, isothiocyanate
moieties can be added on with the desired density
with which scFvs in the form of proteins or, even better,
peptides will be immobilized on the surface of sensor. The
standard protocols of screening with phage display library
or ribosome display library will be briefly discussed.
Speaker Biography
Yaw-Kuen Li received his PhD degree from Tulane University, USA, in
1991. After his postdoctoral research in School of Medicine of Johns
Hopkins University, he moved back to Taiwan to start his academic
career in 1993. He was promoted to a full professor in 2002. Further,
he became the chair of the department in 2004 and the Dean of
college of science in 2014. His primary research interests include
three major fields: (a) Enzyme-based catalytic biological reactions, (b)
Bio-recognition and Bio-sensors, (c) Solid-state/biological interface
chemistry.
e:
Yaw-Kuen Li, Asian J Biomed Pharmaceut Sci, Volume:9
DOI: 10.4066/2249-622X-C2-019
Notes: