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May 13-14, 2019 | Prague, Czech Republic
Chemistry and Medicinal Chemistry
9th World Congress on
Page 55
Asian Journal of Biomedical and Pharmaceutical Sciences | Volume 9 ISSN: 2249-622X
Feng-Huei Lin
National Health Research Institute, Taiwan
Synthesis and biocompatibility of Diamine biomolecules-
functinoalized SWCNTs as vectors for Gene Delivery
The development of new and stable gene carrier is of
great importance to improve the transfection efficiency
and achieve to certain fundamental needs in gene therapy
such as therapeutic protein formation, immunogenicity
enhancement and apoptosis induction. The stability and
applicability of the system that contains single-walled
carbon nanotubes (SWNCTs) to combine with plasmid
DNA (EGFp-C1) was examine by computer simulation.
Raman spectroscopy was used as supporting information
to verify the process and phenomenon of complex
formation. Two diamine biomolecules, 1,4-diaminobutane
and polyoxyethylene bis-amine, were applied in order to
load positive charges onto the surface of SWCNTs under
physiology condition and also enhance the biocompatibility
of SWCNTs. The formation of peptide bond was examined
with FTIR spectroscopy to confirm the result of cross liking.
Concentration of surface functional groups was examined
with TGA, which found that they are at an extremely low
concentration but show great influence on the physical
property. The binding efficiency of functionalized SWCNTs
to EGFp-C1 was analyzed through the binding strength
under electrophoresis. Cytotoxicity and cell viability were
evaluated with LDH and MTT assay that show a significant
increase in cell viability for SWCNTs-1,4-diaminobuatne
complex. The inflammatory inducing property were
assessed by pro-inflammatory factor IL-6 release by using
quantitative sandwich enzyme immunoassay technique.
Speaker Biography
Feng-Huei Lin is expertise in biomaterials, tissue engineering &
regenerative. He obtained his BS degree in Department of Earth
Sciences, National Cheng Kung University (NCKU), Taiwan, in 1980. In
1983, he joined the Functional Ceramics Lab in Institute of Materials
Sciences and Engineering, NCKU, Taiwan. During the PhD training, he
was not only to take course in engineering school, but also in medical
school; that included biochemistry, cell biology, physiology, pathology,
anatomy etc. He was honored as PhD degree with 12 SCI publications
in November 1989. He served as standing committee member in many
international societies since 1996. and as editor, associate editor
and editorial board members in many SCI journals. He has published
over 350 SCI papers, joined 9 book chapters, awarded 78 patents and
transferred 27 technologies to industry to be product. He is willing
to cooperated with different kinds of researchers and scientists; and
very happy to help young blood to do the research by sharing the
knowledge, experience and lab tools. He is very experience both
in academic research and industry to push the research fruits to the
commercial product.
e:
Feng-Huei Lin, Asian J Biomed Pharmaceut Sci, Volume:9
DOI: 10.4066/2249-622X-C2-019
Notes: