17 / 17
Page 42
Notes:
allied
academies
Cell Science, Stem Cell Research &
Pharmacological Regenerative Medicine
November 29-30, 2017 | Atlanta, USA
Annual Congress on
Adv cel sci tissue cul 2017 | Volume 1 Issue 2
Cis-vaccenic acid induces differentiation and up-regulates gamma globin synthesis in K562, JK1 and
transgenic mice erythroid progenitor stem cells
Idowu A Aimola
1, 2
, Hajiya M Inuwa
1
andrew J Nok
1
, Aisha I Mamman
1
and
James J Bieker
2
1
Ahmadu Bello University Teaching Hospital, Nigeria
2
Mount Sinai School of Medicine, USA
G
amma globin induction remains a promising
pharmacological therapeutic treatment mode for sickle
cell anemia and beta thalassemia, however, hydroxyurea
remains the only FDA approved drug which works via
this mechanism. In this regard, we assayed the γ-globin
inducing capacity of Cis-vaccenic acid (CVA). CVA induced
differentiation of K562, JK1 and transgenic mice primary
bone marrow hematopoietic progenitor stem cells. CVA also
significantly up-regulated γ-globin gene expression in JK-1
and transgenic mice bone marrow erythroid progenitor stem
cells (TMbmEPSCs) but not K562 cells without altering cell
viability. Increased γ-globin expression was accompanied by
KLF1 suppression in CVA induced JK-1 cells. Erythropoietin
induced differentiation of JK-1 cells 24 h before CVA induction
did not significantly alter CVA induced differentiation and
γ-globin expression in JK-1 cells. Inhibition of JK-1 and
transgenic mice bonemarrow erythroid progenitor stemcells
fatty acid elongase5 (Elovl5) and Δ9 desaturase suppressed
the γ-globin inductive effects of CVA. CVA treatment failed
to rescue γ-globin expression in Elovl5 and Δ9-desaturase
inhibited cells 48 h post inhibition in JK-1cells. The data
suggests that CVA directly modulates differentiation of JK-1
and TMbmEPSCs and indirectly modulates γ-globin gene
expression in these cells. Our findings provide important
clues for further evaluations of CVA as a potential fetal
hemoglobin therapeutic inducer.
Speaker Biography
Idowu A Aimola is a Senior Academic Staff at the Department of Biochemistry, Ahmadu
Bello University, Zaria. He is also a member of the Africa Centre of Excellence on
Neglected Tropical Diseases and Forensic Biotechnology at the University. He obtained
his PhD in Biochemistry from Ahmadu Bello University, Zaria and was a Fulbright
Scholar at the Department of Cell and Developmental Biology, Mount Sinai School of
Medicine New York. He is a member of the Sickle Cell Support Society of Nigeria. His
main research interest is in red blood cell developmental biology and hematological
disorders. His research work involves studying molecular mechanisms, genetics,
epigenetics and small molecules induction of fetal hemoglobin expression. He has
scholarly publications in renowned peer reviewed international and national journals.
He is an Editorial Member of some international journals.
e:
idowuaimola@gmail.com