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academies
Cell Science, Stem Cell Research &
Pharmacological Regenerative Medicine
November 29-30, 2017 | Atlanta, USA
Annual Congress on
Adv cel sci tissue cul 2017 | Volume 1 Issue 2
First report of citrus yellow mosaic virus from sweet oranges in Pakistan
Shazia Mannan
COMSATS Institute of Information Technology, Pakistan
C
itrus fruits are one of the most important fruits in term
of consumption as well as nutritional value. However,
being such an important crop, it is infected by many
diseases. One of the important diseases that can cause
significant loss is citrus yellow mosaic virus disease. Citrus
yellow mosaic virus causes severe mosaic disease in sweet
oranges and has been reported earlier from India. In 2016,
mosaic symptoms were observed in sweet oranges orchards
in the Okara district at Punjab province of Pakistan. Leaf
samples were collected from the 50 trees with symptoms.
DNA was extracted by using sodium sulphite method.
Conserved region of CYMV was amplified with PCR using
primer pair (5GTGGCTTTCATCAGGTAGC, forward and
5CATGCATCCATCCGTTTCG, reverse). Approximately 640 bp
Product was obtained. The PCR product was sequenced
and BLASTn was performed and 96% similarity was found
with the Indian isolate (GenBank accession no. AF347695).
CYMV particles were propagated and transmitted to
five healthy sweet orange trees through grafting. CYMV
symptoms appeared after 65 days of grafting. After 80 days
of transmission DNA was isolated from leaves and PCR for
CYMV was conducted as described earlier. A Product of
638 bp was obtained. Thus, fulfilling Koch’s postulates. To
our knowledge this is the first report of CYMV in citrus in
Pakistan.
Speaker Biography
Shazia Mannan from the city of Sahiwal which is located in the south of Punjab province
of Pakistan. After completing her graduation from Baha-ud-din Zakariyya University,
Multan, Pakistan. She got her post graduate degree in Biochemistry/Molecular biology
from Quaid-i-Azam University Islamabad, Pakistan in 2001. Then she did her Doctorate
from the same university in Biochemistry/Molecular Biology in 2007. After that she
won Charles Wallace Fellowship of British Council and did her post doctorate research
at Central Sciences Laboratories Yorkshire, UK in 2008. Then she joined department
of Biosciences, COMSATS Institute of Information Technology Sahiwal campus in 2008
as Assistant Professor. Her research interest subjects are Molecular Phytopathology
and drug designing. She has been working on plant pathogens including Xanthomonas
Oryze pv. Oryze, phytoplasma, cotton leaf curl viruses and maize dwarf mosaic virus.
She is also working on computational drug designing against cancers. She is supervising
research of undergraduate and post graduate students at COMSATS Sahiwal campus
e:
shazia@ciitsahiwal.edu.pk