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CANCER STEM CELLS AND
ONCOLOGY RESEARCH
11
th
International Conference on
Journal of Medical Oncology and Therapeutics
|
Volume 3
Sina Taefehshokr et al., J Med Oncl Ther 2018, Volume 3
IN-VIVO
AND
IN-VITRO
IMPACT
OF miR-21 AND miR-126 IN THE
SUPPRESSION OF METASTASIS AND
INVASION IN BREAST CANCER
Sina Taefehshokr
1
, Ehsan Mikaili
1
, Sahand Fattahi
1
, Farzad
Rahimi
1
, Reza Vaezi
1
and
Peyman Keyhanvar
2
1
Islamic Azad University, Iran
2
Iran University of Medical Sciences, Iran
T
he aim of this study was to investigate the effect of miRNA-21 and
miRNA-126 inhibition on metastasis and invasion in both MDA-
MB231, MDA-MB468 as well as the MCF-7 breast cancer cell lines and
five week old female C57BL/6 mice. Following the cloning of miRNA-21
and miRNA-126 into vectors, their expressions were determined before
treatment with constructs of miR-21 and miR-126 in cancer cell lines and
normal breast cells. Then miRNA-21 and miRNA-126 were transfected to
the cell-lines and the expression was assessed after 48 hours. Moreover,
levels of migration and invasion were determined in cell-lines. These
experiments performed in five-week old female C57BL/6mice. The results
showed that miRNA-21 expression before the transfection of miRNA-21
construct was decreased 4, 70 and 100 times in MCF-7, MDA-MB468 and
MDA-MB231 cell lines, respectively, in comparison to normal breast cells;
but after the transfection of miR-21 construct, expression of miRNA-21
was increased 100 times. Furthermore, invasion and migration decreased
by 15 and 10 times in MDAMB-468. All modifications in miRNA-126 were
low in comparison to miRNA-21. The results of the
in vivo
experiments
were approximately the same as
in vitro
experiments. It suggests that
the use of miRNA-21 is highly efficient than miRNA-126 in the inhibition
of metastasis and invasion in breast cancer. Our study enhanced our
conception about miR-21 and miR-126 and its roles in identification and
therapy of breast cancer.
Sina Taefehshokr is a self-motivated, dedicated
and first top DVM student in the faculty with
solid background in Cell Biology, Oncology and
Immunology research. She has published more
than 15 papers both in Farsi and English in sev-
eral journals. She had six both oral and poster
presentations in international and national con-
ferences. She can implement several research
skills including Immunology, Molecular Biology,
Tumor, Protein, Histology and Animal Handling
Techniques. She has worked as a Lab Demon-
strator/Teaching Assistant and Honorary Re-
search Assistant at Stem Cell and Regenerative
Medicine Institute, Tabriz University of Medical
Sciences.
Sinataefehshokr@gmail.comBIOGRAPHY