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CANCER STEM CELLS AND
ONCOLOGY RESEARCH
11
th
International Conference on
Journal of Medical Oncology and Therapeutics
|
Volume 3
Corinne Auge Gouillou et al., J Med Oncl Ther 2018, Volume 3
SETMAR IN GLIOBLASTOMA: SPLICE
VARIANTS AND FEEDBACK NETWORK
IN CONTROLLING TARGET GENES
EXPRESSION
Corinne Auge Gouillou
1
, Sylvaine Renault
1
, Jérôme Jaillet
1
,
Murielle Genty
1
, Edouard Coudert
1
, Oriane Lié
1
and
Ilyess
Zemmoura
2
1
University of Tours, France
2
CHRU of Tours, France
S
ETMAR is a chimeric protein, acting as a house-kipping genome
guardian in healthy cells. In a recent work [i], we demonstrate that
SETMAR expression increases in GBM where different splice variants are
produced depending on the stage of the cells: stem cells express a small
hyper-stable SETMAR (sm-SETMAR) whereas differentiated cells express
a large formknownas the “regular”SETMARenzyme (r-SETMAR).Theonly
difference between both SETMAR proteins originates from the lack of the
SET domain on the sm-SETMAR, due to exon-exclusion during pre-mRNA
maturation. As a result sm-SETMAR is devoid of any methyl-transferase
activity, preventing chromatin modifications and regulations usually
assign to r-SETMAR. In contrast, both proteins are still able to promote
DNA repair by NHEJ, albeit sm-SETMAR is less effective. Our current
works hypothesis that sm-SETMAR may contribute to confer cancer stem
cells properties of chimio- and radio-resistance, in addition to alter their
normal epigenetic profile. Because SETMAR originates from a mobile
genetic element, the human genome contains of thousands of SETMAR
DNA binding sites that are in fact fossils of the original transposon. They
together constitute a regulatory network. The characterization of target
genes differentially regulated by the one or other one of the SETMAR
proteins through this network during GBM biogenesis is under progress.
Corinne Auge Gouillou has completed her PhD
at the Pasteur Institute of Paris in 1993 and
postdoctoral studies from Tours University. She
has been leading her research team for over 15
years and published more than 25 papers in re-
puted journals. She is very strongly involved in
teaching and pedagogy, especially for young
students who arrive at the University. She has
been serving as a referee for many journals, and
led a French network dedicated to mobile DNA.
auge@univ-tours.frBIOGRAPHY