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N o v e m b e r 2 1 - 2 2 , 2 0 1 8 | M a d r i d , S p a i n
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Analytical Chemistry 2018 Journal of Chemical Technology and Applications| Volume 2
ANALYTICAL CHEMISTRY
International Conference on
Kozitsina A et al., J Chem Tech App 2018, Volume 2
ORIENTED IMMOBILIZATION OF
ANTIBODIES ON THE ELECTRODE SURFACE
FOR IMPROVING THE ANALYTICAL
CHARACTERISTICS OF ELECTROCHEMICAL
IMMUNOSENSORS
Kozitsina A, Malysheva N, Svalova T, Medvedeva M
Saigushkina A and Bausheva A
Ural Federal University, Russia
The electrochemical method of detection associated with specific immu-
nochemical reactions occurring on the working area of the transducer is
extremely attractive. Electrochemical immunosensors are characterized by
simplicity, reliability, relatively low cost, the possibility of achieving low de-
tection limits, simplicity of automation and miniaturization, small operating
volumes.
Method of immobilization of antibodies on the working surface of the sensor
played a key role in creating of immunosensors.
The robust and oriented immobilization of the bioreceptor increases the sen-
sitivity and accuracy of detection, and also allows the regeneration of the
surface, thereby increasing the life of the sensor. Immunosensors developed
using covalent immobilization of antigens/antibodies are characterized by
higher accuracy and reproducibility.
The search for new ways of targeted immobilization of protein receptors on
the transducer surface using “friendly linkers“ is an extremely urgent task to-
day, since it allows for a short time to provide the bioreceptor immobilization
in aqueous solutions with physiological pH and temperature. Such immobili-
zation methods do not degrade protein structures that reduce their recogni-
tion ability, and allow multiple reuses of antigen / antibody and greater variety
of immunosensors designs.
Two methods of immobilizing antibodies on the transducer surface are pro-
posed:
1. Electrochemical assisted copper-catalyzed azide-alkyne cycloaddi-
tion using copper nanoparticles.
2. Electrografting of 5-diazo-1H-[1,2,4]triazole-3-carboxylic acid fol-
lowed by antibody immobilization by carbodiimide cross-linking.
Carcino-embryonic antigen/antibody (CEA) was used as amodel. Theworking
electrode was a glass-carbon disc (Metrohm). The electrochemical response
was detected voltammetrically and using the electrochemical impedance
spectroscopy method (mediator system was K4[Fe(CN)6] / K3[Fe(CN)6]).
The obtained results demonstrate the high strength and stability of biorecep-
tor immobilization in comparison with the physical sorption.
Kozitsina A has completed her PhD from Ural State Uni-
versity named after A.M. Gorkiy (Yekaterinburg, Russia).
She is a head of department of analytical chemistry Insti-
tute of Chemical Engineering of the Ural Federal Univer-
sity named after the first President of Russia B.N.Yeltsin.
She has published more than 30 papers in reputed jour-
nals.
BIOGRAPHY