3 / 9
Page 29
Note:
N o v e m b e r 2 1 - 2 2 , 2 0 1 8 | M a d r i d , S p a i n
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
Analytical Chemistry 2018
Journal of Chemical Technology and Applications
|
Volume 2
ANALYTICAL CHEMISTRY
International Conference on
Ivanova A V et al., J Chem Tech App 2018, Volume 2
STUDY OF ANTIOXIDANT PROPERTIES OF
COMBINED VITAMIN PREPARATIONS BY
POTENTIOMETRIC METHOD
Ivanova A V, Gazizullina E R , Timina D S , Borisova M V
and
Gerasimova E L
Ural Federal University, Russia
T
he oxidative action of reactive oxygen species (ROS) in the human body
can lead to disruption of cell membranes, and thus cause a number of
diseases. Compounds with antioxidant properties of endogenous and exoge-
nous nature carry out regulation of the oxidation processes. Since all process-
es take place in the body both in the aqueous and in the lipid phase, in order to
maintain the redox balance of the organism, the use of combined pharmaceu-
ticals containing active substances, both lipophilic and hydrophilic in nature,
is most effective, and therefore it is relevant to study the antioxidant proper-
ties of the pharmaceuticals. Earlier, we developed the potentiometric meth-
od for studying antioxidant properties using the K3[Fe(CN)6]/K4[Fe(CN)6]
system as an oxidizer model1. However, the limitation of the potentiomet-
ric method is the low solubility of the complexes in lipophilic media, which
makes it impossible to evaluate the antioxidant properties of the important
lipophilic antioxidants, for example, α-tocopherol. The aim of this work was
to study the total antioxidant capacity (TAC) of different hydrophilicity anti-
oxidants mixtures by the example of vitamins C and E, which are important
components of metabolism and are part of many vitamin preparations. The
studies were carried out in a water-emulsion medium in the presence of dif-
ferent type’s surfactants. Various surfactant concentrations (0.5-2 mM) and
concentrations ratio of surfactant:α-tocopherol (1:1-5:1) were studied. The
criteria for selecting surfactants were stability of the system potential, ad-
herence to the Nernst dependence, the emulsion stability. The nonionic sur-
factants Triton X-100 and Brij 35 satisfy the criteria. TAC model mixtures of
vitamins C and E in different concentrations and commercial vitamin prepa-
rations containing C and E with the use of selected surfactants were studied.
The experimental data obtained confirm the possibility of using the proposed
approach for studying TAC mixtures of different hydrophilicity antioxidants.
The relative standard deviation does not exceed 5-7%. 1Kh. Z. Brainina, A. V.
Ivanova, E. N. Sharafutdinova,E. L. Lozovskaya, E. I. Shkarina “Potentiometry
as a method of antioxidant activity investigation” Talanta, 71 (2007), p.13-18.
Ivanova A V has completed her PhD from Ural State
University of Economics (Yekaterinburg, Russia). She
is associate professor of the department of analytical
chemistry, Institute of Chemical Engineering of the Ural
Federal University named after the first President of Rus-
sia B.N.Yeltsin. She has published more than 25 papers
in reputed journals.
a.v.ivanova@urfu.ruBIOGRAPHY