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N o v e m b e r 2 1 - 2 2 , 2 0 1 8 | M a d r i d , S p a i n
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
Analytical Chemistry 2018
Journal of Chemical Technology and Applications
|
Volume 2
ANALYTICAL CHEMISTRY
International Conference on
Vachnadze N et al., J Chem Tech App 2018, Volume 2
STUDY ON CYTOTOXIC ACTIVITY OF
SOME EXTRACTS OF
VINCA ROSEA L
.
INTRODUCED TO WESTERN GEORGIA
Vachnadze N, Vachnadze V, Mshvildadze V, Legoult J
and
Pichette A
TSMU I. Kutateladze Institute of Pharmacochemistry, Georgia
M
alignant cancer belongs to the number of the most widespread diseases
in the world. As WHO forecast notifies number of victims of malignant
cancer will be 2015 will die 9 million, 11,4 million in 2030. So mortality caused
by the malignant tumors is presented disturbing statistical data.
The object of our research were the alkaloids extracts obtained from aerial
parts of Vinca rosea L. Cytotoxicity was assessed against A-549 (lung can-
cer cell line), DLD-1(colon adenocarcinoma cell line) and WS-1 (normal skin
fibroblast cell line) cytotoxic activity was expressed inhibiting cell growth by
50% (IC50). Etoposide was used as positive control.
Results:
In vitro
cytotoxic activities of extracts 1,2 were assessed against us-
ing resasurin reduction test, but extract 3 Hoechs test.
Table
in vitro
cytotoxic activities of extracts 1,2,3
Compounds
Resasurin
Hoechs
Cell lines
Cell lines
A-549
mg/ml
DLD-1
mg/ml
WS-1
mg/ml
A-549
mg/ml
DLD-1
mg/ml
WS-1
mg/ml
extracts 1 1,563
1,563
8±3
extracts 2 3,7±0,2
3,8±0,4
1±2
etoposide 2,3±0, 2μM 2,8±0, 04μM 19± 3μM
extracts 3 4,6±0,22 3,57±0,08 2,3±0,1 0,45±0,4 2,7±0,1 2±0,2
etoposide
1,5±0,1 μM 3,0±0,3 μM 3,1±0,5 μM
Biological activities of extracts 1, 2, 3 which were obtained from Vinca rosea
L. introduced to Western Georgia. The results showed that the extracts 1, 2, 3
presented strong cytotoxic activities on A-549, DLD-1 and WS-1.
Vachnadze N has completed her PhD at the age of 45
years fromTbilisi State Technical University, Georgia. She
is a principal scientist of TSMU Iovel Kutateladze Institute
of Pharmacochemistry. She has published more than 60
publications in reputed journals. She is a member of or-
ganizing committee of several international conferences
and meetings.
vachnadze_nina@yshoo.comBIOGRAPHY