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N o v e m b e r 2 1 - 2 2 , 2 0 1 8 | M a d r i d , S p a i n
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Analytical Chemistry 2018 Journal of Chemical Technology and Applications| Volume 2
ANALYTICAL CHEMISTRY
International Conference on
Kintsurashvili L et al., J Chem Tech App 2018, Volume 2
BIOLOGICALLY ACTIVE ALKALOIDS
FROM THE ROOTS OF TAXUS BACCATA L.
GROWING IN GEORGIA
Kintsurashvili L, Mshvildadze V and Gorgaslidze N
TSMU I. Kutateladze Institute of Pharmacochemistry, Georgia
Gen. Taxus L. (Fam. Taxaceae) is famous as the sources of the natural cyto-
static medical preparation for Paclitaxel (Taxol), which has a high antican-
cer activity. Taxus baccata L. is the only one species of Taxus, which is wide-
spread in Georgia. Cytotoxic activity of the alkaloids from the bark and the
leaves of T. baccata was studied “in vitro” tests, using the cells: A-549 (lung
carcinoma), DLD-1 (intestinal adenocarcinoma), WS-1 (human fibroblasts).
The aim of the research was to study content of Taxol and other alkaloids
in roots of Taxus baccata. For deriving of the alkaloids from the row mate-
rial was used the method of liquid-liquid extraction. Based on experimental
researches, the main alkaloids are taxol and karakoline in the sum of alka-
loids from the roots of Taxus baccata grown in Georgia. The cytotoxic activity
of the taxol containing alkaloids, obtained from the roots of Taxus baccata,
was studied at the department of fundamental sciences of the University of
Quebec at Chicoutimi (Canada). Cytotoxic activity of the alkaloids was stud-
ied “in vitro” tests, using the cells: A-549 (lung carcinoma), DLD-1 (intestinal
adenocarcinoma), WS-1 (human fibroblasts). On the base of the researchers
is shown that the substance reveals 50% inhibition of cancer cell cultures:
A-549 (lung carcinoma), DLD-1 (intestinal adenocarcinoma), WS-1 (human
fibroblasts). Standard was etoposide.
Cytotoxic activity of alkaloids from the roots of Taxus baccata L.
Plant
Vegetative
organ Alkaloids
Tumor cells cultures and methods
Resazurine Hoechst
A-549
μg/ml
DLD-1
μg/ml
WS-1
μg/ml
A-549
μg/ml
DLD-1
μg/ml
WS-1
μg/ml
T. baccata roots Taxol,
Karakoline 5±1 5±2 112±10 <1,563 2,4±0,6 9±3
Etoposide 24±4 10±2 35±16 2,1±0,3μМ 2,1±0,3μМ 31±14μМ
Cytotoxic studies show, that the sum of alkaloids from the roots of Taxus bac-
cata shows specific cytotoxic activity in tumor cells: A-549 (lung carcinoma),
DLD-1 (intestinal adenocarcinoma), WS-1 (human fibroblasts).
Kintsurashvili L has completed her PhD at the age of 39
years from I. Kutateladze Institute of Pharmacochemistry
(Georgia). She is a senior scientist of TSMU Iovel Kutate-
ladze Institute of Pharmacochemistry. She has published
more than 80 publications in reputed journals, the author
of 2 patens. She is a member of organizing committee of
several international conferences and meetings.
BIOGRAPHY