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Archives of Industrial Biotechnology | Volume 2
May 14-15, 2018 | Montreal, Canada
World Yeast Congress
M
ushrooms have been widely studied for their
production of extracellular enzymes with lignin-
degrading ability. Enzymes are biological catalysts of great
importance at cellular level, but also, they are of great
interest at industrial level since they are key for certain
reactions to occur, as they increase the rate of reactions
without changing the equilibrium. Typically, enzymes are
produced during fermentation processes of microorganism.
However, low efficiencies and high costs are usually
associated with their production. Therefore, bioprospecting
for new microorganism for the production of enzymes is an
important topic of research. In particular, the basidiomycete
Humphreya coffeata
, a native white-rot fungus found in
Colombia, has not been largely explored for its ability to
produce biologically active metabolites. Given the natural
growth conditions of this fungus, it is likely that lignin-
degradative enzymes are produced, such as: pectinases,
amylases, laccases and cellulases. With this project, we aim
at exploring the ability of
H. coffeata
to produce these four
lignin-degradative enzymes under submerged fermentation
conditions. First, the effect on fungal biomass and enzymatic
production were assessed using two different flasks
geometries and four different growth media, according to
literature reports. Fungal biomass production was measured
by dry weight, while enzymatic activity was determined
using specific protocols depending on the kind of enzyme
that wanted to be evaluated. In general, the enzymatic
extract of each medium was added to a substrate solution,
depending on the evaluated enzyme and either change in
viscosity or absorbance values were recorded. The results of
these evaluations showed that the geometry of the flask did
not affect enzymatic production. On the other hand, greater
enzymatic activities were found for pectinases and cellulases
than for amylases and laccases. In fact, for the latter enzyme,
we have not been able to determine the enzymatic activity
under submerged fermentation; even after evaluating the
addition of different waste/by-products of food industry to
the culture media. However, we found that when adding
ABTS-like inductor under solid fermentation conditions,
some degradation occurred, suggesting that laccases were
produced.
Speaker Biography
L Carmona Saldarriaga has completed her under graduation in Process Engineering
from Universidad EAFIT, Medellin, Colombia. Currently, she is pursuing her Master’s
Degree in Engineering and Bioprocesses at the same institution. She enjoys doing
research and has worked on several projects at the University, such as: establishing the
working conditions for the production of a biopolymer from
Auerobasidium pullulans.
She received an award at COLAEIQ Conference in 2016. Other projects she has worked
on are mainly related to other areas such as: biotechnology, materials and chemical
processes. Currently, she is an Assistant Researcher for the company Cementos Argos
S.A.S, at the Alternative Materials Department.
e:
lcarmon3@eafit.edu.coProjections for the production of enzymes of industrial interest by
Humphreya coffeata
L Carmona Saldarriaga
and
L Marín Palacio
EAFIT University, Colombia