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Archives of Industrial Biotechnology | Volume 2
May 14-15, 2018 | Montreal, Canada
World Yeast Congress
P hosphorylation and dephosphorylation of the checkpoint
kinase CaRad53 is crucial for fungal cells in response to
genotoxic stresses. The catalytic subunit CaPph3 of protein
phosphatase 4 (PP4) forms a complex with the regulatory
subunit CaPsy2, which dephosphorylates activated CaRad53
during adaptation to and recovery fromMMS-mediated DNA
damage. We show here that the N-terminal Y33A mutation
of CaPsy2 blocks the interaction between CaPph3 and
CaRad53, the deactivation of CaRad53 and the morphologic
switch in recovery from genotoxic stress. In Saccharomyces
cerevisiae, the ScPph3-ScPsy2-ScPsy4 complex functions
to dephosphorylate γH2A. Here, we also show that CaPsy4
is a functional homolog of ScPsy4, but not involved in the
deactivation of CaRad53 or CaHta, the ortholog of H2A.
However, deletion of CaPSY4 causes C. albicans cells a
sensitivity to genotoxic reagents and a defect in DNA
damage-induced filamentation. In S. cerevisiae, ScTip41 and
ScTap42 are two regulators of CaPph3. In C. albicans , we
show that deletion of CaTIP41 causes cells to be sensitive
to DNA damaging agents, MMS and cisplatin. In addition,
cells lacking CaTIP41 show a delay in the recovery from
MMS-induced filamentation to yeast form, decreased total
PP2A activity and a defect in deactivation of CaRad53 during
recovery from DNA damage. We also show that CaTip41
interacts with CaPph3, CaPsy2 or CaTap42. And deletion of
CaTIP41 promotes the interaction between CaTap42 and
CaPph3. Finally, C. albicans cells lacking CaPPH3, CaPSY2,
CaPSY4 or CaTIP41 and CaTAP42, and the cells carrying the
Y33A mutation of CaPSY2, show increased virulence to mice.
Therefore, CaPph3 and its regulators play negative roles in
regulating the DNA damage-induced filamentation and the
virulence in C. albicans.
Speaker Biography
Jinrong Feng has completed his PhD from Tianjin University, China. Currently, he is an
Associate Professor at School of Medicine, Nantong University. He has mainly focused
on checkpoint related protein phosphatases in Candida albicans. He has published
over 10 papers in reputed journals.
e:
The roles of checkpoint related protein phosphatases and regulators in regulating morphogenesis and
virulence in Candida albicans
Jinrong Feng
Nantong University, China