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allied
academies
Archives of Industrial Biotechnology | Volume 2
May 14-15, 2018 | Montreal, Canada
World Yeast Congress
N
itrate pollution is caused through the introduction of
excessive amount of nitrates in to surface and ground
water mainly as a result of agricultural activities, human
wastes or industrial pollution. Many industries such as
nuclear industry, food processing, fertilizers, alcohol and
metallurgy generates effluents with high concentration of
nitrates. Nitrates are soluble in water and nitrate polluted
water causes serious environmental and health issues. This
study investigates the nitrate removal by heterotrophic
denitrification process in Sequencing Batch Reactor (SBR).
This process has been chosen because of its simplicity
and higher efficiency for denitrification of wastewater. A
facultative microorganism was isolated from NITK hostel
septic tank waste water and screened under aerobic as well
as anoxic conditions. To isolate a facultative bacterium from
the sample, primary screening was done after enriching the
culture. Further screening was done to identify a efficient
denitrifying bacteria. The efficient bacterial strain (S26)
was identified as Bacillus pumilus by 16S rRNA genome
sequencing method. The effect of various parameters
influencing denitrification by
Bacillus pumilus
was studied.
As a result, denitrification could be obtained at PH 7-7.5,
incubation temperature of 35- 40ºC with carbon source as
acetate. Kinetics studies for denitrification were conducted
and biomass yield at different nitrate concentrations were
experimented and maximum specific growth rate of 0.074/h
was observed during the 6
th
hour of exponential phase.
In future bionitrification experiments to be conducted in
Sequencing Batch Reactor to study the effect of influent
nitrate concentration and effect of carrier loading.
e:
anushya.ashokan@gmail.comStudies on biological denitrification using sequencing batch reactor
Jitendra Pal
and
Anushya A
National Institute of Technology Karnataka, INDIA