8 / 22
Page 47
allied
academies
Archives of Industrial Biotechnology | Volume 2
May 14-15, 2018 | Montreal, Canada
World Yeast Congress
P
resent investigation is elucidating the bioremediation
potential and cadmium-induced cellular response with
its molecular basis in
Candida tropicalis
3Aer. Spectroscopic
analysis clearly illustrated the involvement of yeast cell wall
components in biosorption whereas bioaccumulation was
confirmedbyTEM, SEMandEDXscrutiny. TEMimagesdivulged
extracellular as well as cytoplasmic and vacuolar cadmium
nanoparticle formation, further validated by presence of
ycf1
gene and increased biosynthesis of GSH under cadmium
stress. Transcriptomic and proteomic approaches have rarely
been applied to study change in cell architecture under
environmental stress conditions, but this study is unveiling
the altered expression of proteins and genes in
C. tropicalis
3Aer under cadmium stress in concentration and time
dependent manner, respectively. Fourteen proteins exhibited
differential expression and found involve in cellular redox
homeostasis, nitrogen metabolism, nucleotide biosynthesis
and carbohydrate catabolism. Interestingly, C.
tropicalis
3Aer
is additionally equipped with nitrile hydratase enzyme, rarely
been reported in yeast and thus have potential to remove
nitriles (extremely toxic compounds) from environment. Cd
+2
toxicity not only caused growth stasis but also upregulated
the cysteine biosynthesis, protein folding and cytoplasmic
detoxification response elements.
e:
rehman_mmg@yahoo.comCD
+2
resistance mechanism in
Candida tropicalis
3Aer isolated from industrial effluents
Abdul Rehman
and
Zaman Khan
University of the Punjab, Pakistan