15 / 17
Page 32
Notes:
allied
academies
Virol Res J 2017 Volume 1 Issue 3
International Virology Conference
October 30-31, 2017 | Toronto, Canada
Diversity of geminivirus associated alphasatellites from different cultivated and non-cultivated plants in
Pakistan
Fakhra Shamim
1, 2
, Muhammad Shafiq, Misha Jabbar, Tehmina Bahar
and
Muhammad Saleem Haider
1
The Islamia University of Bahawalpur, Pakistan
2
University of the Punjab, Lahore, Pakistan
T
he viruses belonging to the genus
Begomovirus
are
whitefly transmitted monopartite or bipartite viruses. The
monopartite
begomovirus
es with their associated satellite-
like molecules Alphasatellites are believed to be involved in
enhancing the replication of virus and have a major role in
breaking host defence. The study presented here involved
scanning of different cultivated and non-cultivated plants to
detect and isolate new complexes. Samples of leaves were
collected from different areas of Punjab, Pakistan and screened
for presence of DNA-A, alphasatellite and betasatellite
molecules throughPCR. A total of 8alphasatellite, 5betasatellite
and 1 DNA-A full length genome was reported. Phylogenetic
studies of alphasatellite molecules were made. 6 sequences
isolated from cotton leaves collected from Vihari had close
resemblance of up to 90 % similarity with satellite molecules
of PaLCuA (
Papaya leaf curl alphasatellite
) and could be new
strains of PaLCuA first time reported from cotton plants. The
sequence MJ-24[CLCuMA-PK-Multan-cotton] reported from
Multan displayed 98% similarity with CLCuMA (
Cotton leaf curl
Multan alphasatellite
) indicating that it is a variant of CLCuMA.
MJ-25[GDSA-PK-Multan-cotton] also reported from Multan
was classified as a new strain of GDSA (
Gossypium dawanii
symptomless alphasatellite
) with a score of 93% similarity.
Phylogenetic tree further confirmed the results. An analysis
of ORF with Rep coding sequence of alphasatellites was made
by comparison with earlier reported protein sequences in the
database. Results revealed highly conserved regions of Rep
domain and helicase binding domain. Only one sequence MJ-
25 displayed some substitutions in the Rep domain. Other
substitutions were observed in central region or hydroxyl end
of protein.
Speaker Biography
Fakhra Shamim is currently associated with The Islamia University of Bahawalpur,
Pakistan and University of the Punjab, Lahore, Pakistan.
e:
fakhrasiub@gmail.com