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Virol Res J 2017 Volume 1 Issue 3
International Virology Conference
October 30-31, 2017 | Toronto, Canada
Infectivity and host pathogen interaction study of Chickpea chlorotic dwarf virus L strain isolated from cotton
Muhammad Saleem Haider
1
, Muhammad Shafiq, Fakhra Shamim, Muhammad Tariq Manzoor
and
Fasiha Qureshi
1
University of the Punjab, Pakistan
C
otton leaf curl disease in the Indian subcontinent is
associated with several distinct begomoviruses that interact
with a disease-specific DNA satellite named Cotton leaf curl
Multan betasatellite (CLCuMB). However, we have recently
reported the distinct strain (L) of leafhopper transmitted
chickpea chlorotic dwarf virus, CpCDV-L (genus Mastrevirus,
family Geminiviridae) from cotton plants affected by leaf curl
disease in a small number of plants. The question as to whether
CpCDV-L contributes to the development of disease symptoms
such as leaf curling and enations remain to be answered.
Standard methods were used to produce partial direct and
tandem repeat constructs of CpCDV-L for
Agrobacterium
-
mediated inoculation in the binary vector pBIN19. The role
of CpCDV-L in the induction of typical disease symptoms was
studied by
Agrobacterium
-mediated inoculation of the partial
repeat construct to
Nicotiana benthamiana
. CpCDV-L induced
downward leaf curling leading to cup shape in
N. benthamiana
.
The complete tandem dimeric construct of the virus was also
found to be highly infectious to chickpea, and induced severe
stunting of the plant, leaf smalling, drying, and the eventual
death of the plant. This strain could be future possible threat to
cotton crop in Pakistan.
Speaker Biography
Muhammad Saleem Haider has completed his PhD at the age of 32 years from
University of London, Imperial College of Science,Technology and Medicine, London
(United Kingdom) and Post Doctorate from the University of Toronto, Canada. He is
the director of the Institute of Agricultural Sciences, University of the Punjab, Lahore-
Pakistan. He has published more than 100 papers in reputed journals and has been
serving as an editorial board member of well reputed journals. Currently, also holding
the office of the President, Pakistan Phytopathological Society.
e:
haider65us@yahoo.com