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Virology Research Journal
Volume 1 Issue 4 Vaccines World 2017
Notes:
Page 52
November 09-10, 2017 Vienna, Austria
21st World Congress and Exhibition on
VACCINES, VACCINATION & IMMUNIZATION
Antibody against synthetic peptide of prolactin-
inducible protein homologue precursor (PIP-HP)
of Bali cattle (Bos javanicus) saliva: A potential
biomarker for immunoassay development
Sulaiman N Depamede and Djoko Kisworo
University of Mataram, Indonesia
Background: We have successfully identified sequence of a
Prolactin-Inducible Protein Homologue Precursor (PIP-HP)
in Bali cattle (Bos javanicus ) saliva by matrix-assisted laser
desorption ionization-time-of-flight-mass spectrometry
(MALDI-TOF-MS). PIP-HP as one of salivary proteins that
might potentially altered in disease and could serve as novel
diagnostic biomarkers. Antibody against PIP-HP hence needs
to be produced.
Methods: A synthetic amino acid sequence of the PIP-HP
was developed and then conjugated to bovine serum albumin
and was used to immunize Indonesian local rabbits. Serum
antibody that specific to the PIP-HP was purified sequentially
by ammonium sulfate precipitation and protein A affinity
methods. Purified antibody was then used to analyze the
presence of PIP-HP in the ruminants’ saliva by means
of dot blot and western blot. A preliminary study on the
development of immuno lateral assay using the purified anti-
antibody as the biomarker has also been carried out.
Results: Specific antibody was successfully produced against
a synthetic amino acid sequence fragment of PIP-HP of Bali
cattle saliva. The antibody can be used to analyze the presence
of PIP-HP not only in the saliva but also in other fluids
including semen fluids of large ruminants (cattle and buffalo).
The antibody was also able to be used to develop immuno
lateral assay kits. In addition, we also found that the antibody
was able to inhibit the growth of E. coli culture, but did not
to S. aureus. The findings in this study need to be explored
further especially in the relationship with the potential of
anti-PIP-HP as biomarker for animal diseases biomarker.
Virol Res J 2017, 1:4