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Virology Research Journal
Volume 1 Issue 4 Vaccines World 2017
Notes:
Page 50
November 09-10, 2017 Vienna, Austria
21st World Congress and Exhibition on
VACCINES, VACCINATION & IMMUNIZATION
Dendrimeric peptides can confer protection
against foot-and-mouth disease virus in cattle
Patricia Zamoranoa1, Ivana Soriaa1, Valeria Quattrocchia, Cecilia
Langellottia1, Mariela Gammella2 , Sebastian Digiacomoa, Beatriz Garcia
de la Torrec, David Andreuc, Maria Montoyad, Francisco Sobrinoe and
Esther Blancof
1Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
2Instituto Nacional de Tecnología Agropecuaria (INTA), Argentina
3Universitat Pompeu-Fabra, Spain
4Pirbright Institute, UK
5Centro de Investigación en Sanidad Animal (CISA-INIA), Spain
Foot-and-mouth disease virus (FMDV) causes a highly
contagious disease in cloven-hoofed animals. A synthetic
vaccine candidate consisting of dendrimeric peptides
harboring two copies of a B-epitope [VP1(136-154)] linked to
a T-cell epitope [3A(21-35)] of FMDV confers protection to
type O FMDV challenge in pigs. Herein, we show in cattle that
novel dendrimeric peptides bearing a T-cell epitope [VP1(21-
40] and two or four copies of a B-cell epitope [VP1(135-
160)] from type O1 Campos FMDV (termed B2T and B4T,
respectively) elicited FMDV specific immune responses
to similar levels to a commercial vaccine. Animals were
challenged with FMDV and 100% of vaccinated cattle with
B2T or B4T were protected to podal generalization. Moreover,
bovines immunized with B4T were completely protected
against FMDV challenge (with no clinical signs), which
was associated with titers of viral neutralizing antibodies in
serum higher than those of B2T group (p<0.05) and levels of
opsonic antibodies similar to those of animals immunized
with FMDV commercial vaccine. Bovines vaccinated with
both dendrimeric peptides presented high levels of IgG1
anti FMDV in sera and in mucosa. When IgA in nasal
secretions was measured, 20% or 40% of the animals in B2T
or B4T groups respectively, showed anti-FMDV IgA titers.
In addition, B2T and B4T peptides evoked similar consistent
T cell responses, being recognized in vitro by lymphocytes
from most of the immunized cattle in the proliferation assay,
and from all animals in the IFN-γ production assay. Taken
together, these results support the potential of dendrimers
B2T or B4T in cattle as a highly valuable, cost-effective FMDV
candidate vaccine with DIVA potential.
Virol Res J 2017, 1:4