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Virology Research Journal

Volume 1 Issue 4

Vaccines World 2017

Notes:

Page 49

November 09-10, 2017 Vienna, Austria

World Congress and Exhibition on

VACCINES, VACCINATION & IMMUNIZATION

Veterinary vaccines: Oriented approaches for

intracellular and extracellular pathogens

Angel Adrian Cataldi

National Institute of Agricultural Technology (INTA), Argentina

Statement of the Problem

: We work on two relevant cattle

pathogens such as

Mycobacterium bovis,

agent of bovine

TB and

Escherichia coli

O157:H7, the main member of the

Enterohemorrhagic

E. coli

(EHEC) group, is the agent of

uremic hemolytic syndrome.

M. bovis

and EHEC are both

zoonotic pathogens. But while M. bovis is pathogenic to

both cattle and humans, producing tuberculosis; EHEC do

not provoke an illness in cattle (being cattle a reservoir) but

causing a severe disease in human infants. In turn,

M. bovis

is an intracellular pathogen and EHEC is not an invasive

pathogen.

Methodology & Theoretical Orientation

: We reasoned

that to combat an intracellular pathogen, we need a live

attenuated vaccine deleted in genes related to the virulence

for that we designed a

M. bovis

wild type deleted in

mce

genes, additionally and for safety, another gene (

phoP

) was

deleted. The vaccine probed to be effective in mice, guinea

pig and cattle experimentally challenged. In the case of

EHEC, we used recombinant proteins (rAgs) from the type

3 secretion systems and sometimes additional proteins. rAgs

were emulsified in an adjuvant that promotes mucosal IgG

response. Animals were challenged with an EHEC inoculum

and a protection reducing the bacterial shedding and the

number of excreting animals was observed.

Conclusion & Significance

: The rational design of vaccines

is based in selecting the adequate approach to induce the

adequate protective immune response for a given pathogen.

In our case, results were promissory and prompt us to improve

the current vaccine formulations to obtain efficient vaccines.

cataldi.angeladrian@inta.gob.ar

Virol Res J 2017, 1:4