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Journal of Clinical and Experimental Toxicology | Volume: 2
December 03-04, 2018 | Dubai, UAE
International Conference on
6
th
International Conference on
Toxicology, Clinical Toxicology & Pharmacology
Recycling & Waste Management
Joint Event
&
Anti-inflammatory properties of Crotoxin, the major component of the South American rattlesnake
Crotalus durissus terrificus
, in murine model of Endotoxemia
Suely Vilela
University of Sao Paulo, Brazil
C
rotoxin (CTX) isolated from the venom of the South
American rattlesnake
Crotalus durissus terrificus
have been reported to present anti-inflammatory and
immunosuppressive properties, mediated by the production
of the anti-inflammatory lipid mediator Lipoxin A4 (LXA4).
Therefore, here we describe the capacity of CTX to modulate
the inflammatory response in endotoxemia model induced
by LPS in mice. Swiss male mice were administrated with CTX
(30μg/Kg, s.c.) or saline, and after 2 hours with LPS (15mg/
Kg, i.p.). After 6 hours of LPS administration, serum and lungs
were collected for inflammatory and organ damage biomarkers
quantification. CTX was responsible for a significant reduction
of the serum inflammatory mediators TNF-α and IL-6, as well
as lung mieloperoxidase (MPO) as indicative of lung neutrophil
accumulation. The levels of heart and liver damage biomarkers
creatine kinase Mb (CK-Mb) and aspartate aminotransferase
(AST), respectively, were also decreased. When animals
were pretreated with Boc-2 (10μg/Kg), an antagonist of
the LXA4 receptor FPR (formyl peptide receptor), the anti-
inflammatory and organ damage prevention induced by CTX
(as observed by the decrase in TNF-α, IL-6, MPO, CK-Mb and
AST) were drastically reduced. In conclusion, the results in
the present work show that CTX is capable of ameliorate the
inflammatory signs of endotoxemia induced by LPS, as well as
the organ damages associated with this event. Also, the anti-
inflammatory effect of CTX is associated with FPR possibly
via LXA4 production. These results strongly indicates CTX
as a potential candidate as an immunotherapeutical agent.
Speaker Biography
Suely Vilela, former president of the University of São Paulo (2005-2009), is a Full Professor
of the Faculty of Pharmaceutical Sciences of Ribeirão Preto- University of São Paulo (FCFRP-
USP), a CNPq grantee of research productivity fellowship - Level 1. Graduated in Pharmacy
(1974) and Pharmacy-Biochemistry (1975) by the School of Pharmacy and Dentistry of
Ribeirão Preto, University of São Paulo. She obtained the Master’s and Doctor’s degrees in
Sciences, Biochemistry area, at theMedical School of Ribeirão Preto of USP, where she also
developed a Post-doctorate training (1990). She has over 114 publications in international
scientific journals. Her currently publication H-index is 28. Currently she is a member of the
editorial board of the Journal of Venomous Animals Including Tropical Disease (JVATiD –
BioMedCentral).HerscientificareaconcernsinAnimalToxinsandVenoms,includingscorpion
and snake venoms, working on biological, biochemical and biophysical properties of toxins.
e:
suvilela@usp.brSuely Vilela
, Toxicology 2018 & Recycling 2018, Volume 2
DOI: 10.4066/2630-4570-C1-003