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Journal of Clinical and Experimental Toxicology | Volume: 2
December 03-04, 2018 | Dubai, UAE
International Conference on
6
th
International Conference on
Toxicology, Clinical Toxicology & Pharmacology
Recycling & Waste Management
Joint Event
&
Vascular endothelial growth factor from
Crotalus durissus collilineatus
venom: Structure and
angiogenic properties
Eliane Candiani Arantes
University of Sao Paulo, Brazil
S
nake venoms display important pharmacological activities,
since they present a variety of components including the
vascular endothelial growth factors (VEGFs). Although VEGF
have already been identified by omics analysis in the
Crotalus
durissus collilineatus
(Cdc) venom, it was not isolated so far.
VEGFs are non-enzymatic homodimers of 20-30 kDa, which
regulates angiogenesis by inducing proliferation, migration
and permeability of endothelial cells. However, their role in
the envenoming pathophysiology is not yet elucidated. In the
present study, a new VEGF fromCdc venomwas isolated and its
structure and angiogenic properties were partially determined.
CdcvenomwasfractionatedbyreversedphaseFPLC(FastProtein
Liquid Chromatography) and all the fractions collected were
submitted to an ELISA assay for VEGF identification. Fraction
24 (positive for VEGF) was submitted to an anion exchange
chromatography (HiTrap QXL column) and the CdcVEGF was
purified. It was reduced, alkylatedwith iodoacetamide, digested
with tripsin, and submitted to mass spectrometry analysis (ESI-
QTOF and PMF), in order to determine its primary structure.
It showed a molecular mass of 25.705 kDa. An angiogenesis
in vitro assay by the induction of tube formation in Matrigel®
by HUVECs cells was performed, showing that CdcVEGF
induced a tube formation even more significant than the
positive control (bFGF). This work was pioneer on the isolation
and partial characterization of a new VEGF from Cdc venom.
Speaker Biography
Eliane Candiani Arantes is Full Professor of School of Pharmaceutical Sciences, University
of São Paulo (USP), a CNPq grantee of research productivity fellowship - Level 1. Graduated
in Pharmacy (1979) and Pharmacy-Biochemistry (1980) by the School of Pharmacy
and Dentistry of Ribeirão Preto, USP. She obtained the Master’s and Doctor’s degrees
in Sciences, Biochemistry area, at the Medical School of Ribeirão Preto of USP, and
developed a Post-doctorate training (2012) at University of Liège, Faculty of Sciences, Mass
Spectrometry Laboratory. She has 110 publications that have been cited over 2500 times,
her publication H-index is 32 and has been serving as an editorial board member of Journal
of Venomous Animals and Toxins Including Tropical Diseases.
e:
ecabraga@fcfrp.usp.brEliane Candiani Arantes
, Toxicology 2018 & Recycling 2018, Volume 2
DOI: 10.4066/2630-4570-C1-003