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J Clin Exp Tox 2017 | Volume 1 | Issue 2

Toxicology and Pharmacology

November 01-02, 2017 | Toronto, Canada

International Conference on

The connection of changed cholinergic receptors with the deficit of learning and memory of rats with

chronic fluorosis

Zhi-Zhong Guan, Yang-Ting Dong, Yan-Jie Liu, Chuan-Zhi Gui, Qin Gao

and

Na Wei

Guizhou Medical University, China

n order to reveal the mechanism of decreased learning

ability and memory induced by chronic fluorosis,

nicotinic acetylcholine receptors (nAChRs) and muscarinic

acetylcholine receptors (mAChRs), cholinesterase (ChE)

activity and oxidative stress were investigated using rats

with chronic fluorosis. Spatial learning and memory of

the rats were evaluated by Morris Water Maze test. The

expressions of nAChRs and mAChRs at protein and mRNA

levels were detected by Western blotting and real-time

PCR, respectively. ChE activity and the level of oxidative

stress were determined by chemical colorimetry. The results

showed that the learning and memory capacity in rats with

chronic fluorosis was decreased. In the brain tissues from

the rats with fluorosis as compared to controls, the protein

expressions of nAChR and mAChR subunits were lower,

and the corresponding mRNAs of these receptor subunits

changed; the activities of acetylcholinesterase (AChE) were

reduced, but no change of butyrylcholinesterase (BuChE);

the increased MDA content and the decreased activities

of SOD and GSH-px were found. The results indicated that

the deficit of learning and memory of the rats with chronic

fluorosis may be in mechanism correlated with the inhibited

expression of cholinergic system and the increased level of

oxidative stress.

Speaker Biography

Zhi-Zhong Guan completed his PhD from Karolinska Institutet, Sweden in 1997. He is

the Director of the Key Lab of the Endemic and Ethnic Diseases in Education Ministry of

China. He has published more than 400 papers (including more than 100 SCI collected

papers) in peer-reviewed journals and has been serving as an Editorial Board Member

or reviewer of several journals.