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J Clin Exp Tox 2017 | Volume 1 | Issue 2
Toxicology and Pharmacology
November 01-02, 2017 | Toronto, Canada
International Conference on
Neuropotential roleofTaurine:Roleofneurotransmitters, oxidativestress,mitochondrial dysfunctioning
and histopathological evidences
Manveen Bhardwaj and Anil Kumar
Panjab University, India
Rationale: Alterations in neurotransmitters levels is the main
culprit of the epilepsy. With the antioxidant effects, taurine
cause the alterations in the Glutamate and GABA levels. But
its mechanism of action in epilepsy is still undeciphered. Thus,
there exists rationale in preventing the glutamate excitotoxicity
by taurine as neuroprotective strategy in pentylenetetrazole
(PTZ) induced kindling epilepsy.
Objective: Aim of present study is to investigate the
neuroprotective role of taurine and its modulation by
minocycline (Mino) in the kindling epilepsy.
Method: PTZ (40mg/kg, i.p.) was administered alternatively for
29daysuntilanimalexhibitedfullmotorseizures.Taurinewasgiven
orally at a dose of 25, 50 and 100mg/kg by dissolving it in distilled
water once a day 1 h prior to PTZ treatment and minocycline at
the dose of 50 and 100 mg/kg and its combination (Taurine 50
mg/kg + Mino50 mg/kg) and (Taurine 100 mg/kg + Mino100 mg/
kg) for the period of 29 days. Various neurobehavioral parameters
followed by biochemical, mitochondrial respiratory enzyme
complexes (I-IV), neurotransmitter examinations (Glutamate,
GABA, Serotonin, Dopamine and Norepinephrine) by HPLC and
histopathological alterations by haematoxylin and eosin stain
were assessed.
Results: PTZ administration significantly impaired the cognitive
performance in the morris water maze (MWM) performance
test, increased the seizure score, caused oxidative stress,
mitochondrial dysfunctioning and also caused alterations
in the neurotransmitter levels and in the histopathology of
hippocampus and cortex. Treatment with the taurine (25,
50 and 100 mg/kg), minocycline (50 and 100 mg/kg) for 29
days significantly improved the seizure score, reduced AChE
activity, oxidative damage (reduced LPO, nitrite level and
elevate the SOD, catalase and GSH levels) and also restored the
mitochondrial complexes (Complex I, II and IV) and improved
the neurotransmitter levels (Glutamate, GABA, Serotonin,
Dopamine and Norepinephrine). Combination of taurine with
minocycline showed more significant effects as compared to
the per se effect. Further, histopathological alterations showed
the significant improvement effects in the combination of
taurine with minocycline.
Conclusion: Taurine when combined with minocycline show
the neuroprotection by decreasing the glutamate excitotoxicity
against the PTZ induced kindling epilepsy.
Speaker Biography
Manveen Bhardwaj is a PhD Research Scholar at Panjab University, Chandigarh, India.
She did her M. Pharmacy I.S.F college of Pharmacy, Moga in the year 2013. She has
been awarded with “Young Scientist Award” at Punjab science congress at SUS college
of Tangori Mohali.
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