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J Clin Exp Tox 2017 | Volume 1 | Issue 2
Toxicology and Pharmacology
November 01-02, 2017 | Toronto, Canada
International Conference on
Ameliorative potential of Eugenol and Carvacrol in cobalt mediated hypercontraction in isolated
Wistar rat aorta
Shahnawaz Ahmad Wani
and
Seemi Farhat Basir
Jamia Millia Islamia, India
C
obalt is a very important element that is naturally
present in biochemically important compounds like
cyanocobalamin (Vit B
12
). But occupational exposure
of cobalt is reported to cause various diseases like lung
cancer, cardiovascular diseases like cardiomyopathy and
gastrointestinal diseases. Cobalt is reported to augment
vascular contractility in spontaneously hypertensive rats.
So, we have planned this study to check contractile effect
of cobalt in toxic range on Wistar rat aortic rings, and to
study the ameliorative potential of eugenol and carvacrol
which are plant derived terpenoids and possess antioxidant
activities using organ bath system (Ad instruments). In
our study, a hyper-contractile response was seen at all
the various concentrations of cobalt used, i.e. 800 nM, 10
µM, and 50 µM and the hypercontractile response in case
of cobalt incubated aortic rings were 132%, 128%, 108%
respectively with respect to control taken as 100% with
Phenylephrine induced contraction. Eugenol and carvacrol
could act as possible ameliorators of hypercontraction.
We have noticed that at saturating concentration of 100
µM eugenol and 10 µM carvacrol caused 38% and 42%
relaxation in cobalt unexposed aortic rings; while 40% and
48% relaxation was observed when cobalt exposed aortic
rings were co-incubated with eugenol and carvacrol. In our
study, we have found that the relaxation caused by both
the natural compounds is due to the quenching of ROS
and by enhancing nitric oxide release from endothelium of
aorta. To conclude, acute exposure of cobalt to aortic rings
causes increase in hypercontractile response by generating
oxidative stress, which is effectively lessened by eugenol and
carvacrol.
Speaker Biography
Shahnawaz Ahmad Wani did his MSc Biochemistry from Jamia Millia Islamia (JMI), New
Delhi India. He is pursuing PhD from Department of Bioscience, JMI and is working on
topic titled as “Effect of Metal Pollutants on Cardiovascular System”. He has presented
four conference paper in various national and international seminars. He has one
publication on titled mechanism of flavonoids in smooth muscle relaxation.
e:
shahnawaz137892@st.jmi.ac.in