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J Clin Exp Tox 2017 | Volume 1 | Issue 2
Toxicology and Pharmacology
November 01-02, 2017 | Toronto, Canada
International Conference on
Safety and toxicity evaluations of
Xanthium strumarium
Linn
Bhanu P S Sagar
and
Srishti Singh
IEC College of Engineering & Technology- IEC Group of Institutions, India
X
anthium strumarium
L. is poisonous to mammals due
its toxic principle which is a diterpenoid glycoside i.e.
atractyloside found in the roots and seeds. It was thought
worthwhile to carry out the hepatotoxic assessments and safety
and toxicity evaluations of oral administration of atractyloside
and methanolic extracts of
X. strumarium
L. in Albino Wistar
rats. So, present investigation was undertaken with following
objectives: To develop standardized protocols for Extraction,
isolation, purification, characterization and quantitative
estimation of Atractyloside; Hepatotoxic assessments of oral
administration of atractyloside in Albino Wistar rats and; To
study the safety and toxicity evaluation of methanolic extract in
Albino Wistar rats.
Xanthium strumarium
Linn. root and seeds
were found to contain alkaloids, anthraquinones, flavonoids,
atractyloside, phenolics, steroids, terpenoids, and resin etc.
In the present investigation, attempt was made to separate
the atractyloside by using instant preparative thin layer
chromatography (IPTLC) technique. Purified atractyloside was
chemically characterized by IR, Mass and NMR spectral analysis.
Atractyloside concentrations were found to be 2.9 and 4.3 mg/
ml in plant root and seeds respectively using HPLC techniques.
During hepatotoxic assessment, atractyloside produced severe
hepatotoxicity in Albino Wistar rats. Observations of the sub-
acute and acute toxicity studies had indicated that methanolic
extract of
X. strumarium
had shown a narrow safety margin
in animals. On the basis of sub-acute and acute toxicity
evaluation studies, it was established that both atractyloside
and methanolic extract of
X. strumarium
L. possess a narrow
safetymargin in rats used in in-vivo experimental and preclinical
pharmacological studies.
Speaker Biography
Bhanu P S Sagar had completed his PhD from Jamia Hamdard, Post-doc from National
Institute of Immunology and DSc in Alternative Medicine. He is presently the Director
of Pharmacy College at IEC-GI & Former Vice-Chancellor of IEC University and has
published 47 papers and presented 30 papers. He has presented two papers in
“AAPS 2006 National Biotechnology Conference” in Boston, USA. He is evaluator for
various international journals and also selected for “Marquis Who’s Who in Asia” and
“Marquis Who’s Who in World”. He has received many awards and prime areas of
research include Plant Tissue Culture, Phytochemical & Pharmacological investigations
of natural products.
e:
bpssagar@gmail.com