stds-stis-hiv-aids-2017-posters-accepted-abstracts

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Page 52
Virology research J 2017 Vol 1 Issue 2
July 26-27, 2017 | Vancouver, Canada
WORLD CONFERENCE ON STDs, STIs & HIV/AIDS
alliedacademies
Every day, more than 1,800 babies worldwide contract
HIV from their mothers. Many of these cases occur in
Africa including Ethiopia. The transmission of HIV from
infected mothers to babies could occur during pregnancy,
delivery and breastfeeding. For women to take advantage of
measures to reduce transmission, they need to know about
Mother To Child Transmission (MTCT) of HIV and their HIV
status. The aim of this study was to assess the knowledge
on MTCT and utilization of services designed for prevention
of mother-to-child transmission (PMTCT) of HIV/AIDS among
pregnant women. Community based cross-sectional study
was conducted at Hossana town from March 3-28/ 2014
using pre-tested questionnaire and structured interviews.
The collected data was analyzed using SPSS version 16.
Out of the 417 pregnant women interviewed, 370 (88.7%)
responded that they knowMTCT of HIV, 377(90.4%) mothers
tested for HIV during current pregnancy and 354(93.9%)
shared test result to their husband. Knowledge of Mother
to child transmission was the independent predictor of
utilization of the services rendered for PMTCT of HIV/AIDS.
More than three-fourth of pregnant women knew about
MTCT of HIV. Nine women in every 10 tested for HIV during
current pregnancy has shared test result to their husband.
Knowledge of mother to child transmission of HIV/AIDS was
the independent predictor of utilization of PMTCT services.
Thus, improving awareness of pregnant women about MTCT
of HIV/AIDS and its prevention strategies by means of health
care providers in maternal and child health service units
should be strengthened.
e:
Knowledge on mother to child transmission and utilization of services designed to prevent mother to
child transmission of HIV/AIDS among pregnant women in Hossanna Town, Southern Ethiopia
Fregenet Zekiewos, Tigist Workneh and Belayneh Hamdela
Wachemo University, Ethiopia
Actively replicative endogenous retroviruses (HERVs) are
anciently integrated in the human genome and became
stable in inherited material across generations, accounting
for 8% of the human genome. Among HERVs group there are
the HERV-K and HERV-W. HERV-K virions were seen budding
from tumor cells, and viral RNA could be found at higher
titers in the plasma of lymphoma and breast cancer patients.
Reverse transcriptase and retroviral RNA were detected in
plasma samples that simultaneously had HERV-K gag and env
proteins, inbothmature andpseudoparticles. In light of these
findings, we propose to initiate a study to evaluate the role of
HERV-K among cancer patients in Brasilia, DF, Brazil. After the
study approval (number 59/13) by the Committee of Ethics,
under patients consent, blood samples and neoplastic tissue
fragments were collected, in the surgery unit of the Hospital
de Base do Distrito Federal. All the patients had cancer in
the neck or head. 35 extracted DNA samples from different
histopathological types which were amplified with primers
to the HERV-K gag region, and 31 samples yield an amplicon
band size of 250 bp, as expected to the gag sequence. There
are substantial reports in the literature of HERV-K proviral
sequences detection among head and neck cancer and also
in healthy tissues. Of 35 analyzed samples, 10 patients with
oral squamous cell carcinoma were previously negative for
the presence of human papillomavirus gene sequences,
but had HERV-K sequences detected. Further analysis will
investigate the presence of other HERV-K sequences as env,
pol and ltr, in order to better understand the role of these
entitites in oncogenesis. Also the obtained amplicons will
be sequenced and compared to published and deposited
sequences.
e:
Detection of HERVs sequence in head and neck cancer patients in a public hospital (HBDF) in Brasilia,
DF, Brazil
L I B Kanzaki1, Laís S Mello1, Thatiana L Sampaio1, Marcus Vinicius V Coimbra1, Elida Cleyse G Da mata1, Cláudio C Cavalcanti2, Sonia N Bao1, Elisiário C
Leitão3, Rondinelly R Ribeiro2, Ernani Kaoru Nakamura2, Marcelo O P Cavassani2 and Francisco Mitrovick2
1University of Brasilia, Brazil
2Hospital de Base, Brazil
3HRAN, Brazil