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April 15-16, 2019 | Frankfurt, Germany
Applied Physics & Laser, Optics and Photonics
International Conference on
Materials Science and Nanotechnology | Volume: 3
Multi-photon near-infrared emission saturation nanoscopy using upconversion
nanoparticles
Fan Wang, Chaohao Chen, Shihui Wen, Qian Peter Su, Mike C L Wu, Yongtao Liu, Baoming Wang, Du Li,
Xuchen Shan, Mehran Kianinia, Igor Aharonovich, Milos Toth, Shaun P Jackson, Peng Xi
and
Dayong Jin
University of Technology Sydney, Australia
M
ultiphoton fluorescence microscopy (MPM),
using near infrared excitation light, provides
increased penetration depth, decreased detection
background and reduced phototoxicity. Using
stimulated mission depletion (STED) approach,
MPM can bypass the diffraction limitation, but
it requires both spatial alignment and temporal
synchronizationofhighpower(femtosecond)lasers,
which is limited by the inefficiency of the probes.
Here, we report that upconversion nanoparticles
(UCNPs) can unlock a new mode of near-infrared
emission saturation (NIRES) nanoscopy for deep
tissue super-resolution imaging with excitation
intensity several orders of magnitude lower than
that required by conventional MPM dyes. Using a
doughnut beam excitation from a 980 nm diode
laser and detecting at 800 nm, we achieve a
resolution of sub 50 nm, 1/20th of the excitation
wavelength, in imaging of single UCNP through 93
μm thick liver tissue. This method offers a simple
solution for deep tissue super resolution imaging
and single molecule tracking.
e
:
fan.wang@uts.edu.au