pediatric-healthcare-pediatric-infections-2017-posters

22 / 25

Page 66
alliedacademies
September 20-22, 2017 | Toronto, Canada
10 TH AMERICAN PEDIATRICS HEALTHCARE &
PEDIATRIC INFECTIOUS DISEASES CONGRESS
Pediatric Healthcare & Pediatric Infections 2017
We checked the role of regulatory T cells (Tregs) on the
liver inflammatory response in a lipopoly- saccharide
(LPS)-induced preterm birth mouse model. The LPS-induced
preterm birth mouse model was established. Before LPS
treatment Tregs were insulated from pregnant mice and
inoculated into different pregnant mice. The expression of
Hemeoxygenase-1 (HO-1), fork head family transcription
factor (Foxp3), and interleukin-6 (IL-6) in liver were examined
by real-time reverse transcription polymerase chain reaction
and western blotting.The mRNA and protein expression
levels of, HO-1 and Foxp3 in liver from LPS-treated mice
was considerably reduced equated with the controls, while
the adoptive transfer of Tregs expressively rescinded the
changes in the expression of the above said elements after
LPS treatment. Fascinatingly, the expression of IL-6 in the
liver was meaningfully elevated after LPS treatment, and this
effect was obstructed by the adoptive transfer of Tregs. The
preterm birth was remarkably persuaded after maternal LPS
exposure and affected the expression of Foxp3, HO-1and IL-6
in liver tissue. Furthermore, the adoptive transfer of Tregs
absolutely abolished the changes in the expression of the
above factors after LPS treatment. However, further study is
needed to understand the mechanism of Tregs to prevent
the liver inflammation in preterm birth in human.
e:
The investigation on the protective role of regulatory T cells in lps induced fetliver damage in late
pregnant mice
Muhammad Siddiq, Li Liu
Xi’an Jiaotong University College of Medicine, China