nutrition-world-2017-posters-accepted-abstracts

16 / 29

alliedacademies
Insights Nutr Metab 2017
Volume 1 Issue 3 Nutrition World 2017
Page 66
September 11-12, 2017 Edinburgh, Scotland
15 th World Congress on
Advances in Nutrition, Food Science & Technology
Bioactive fiber: Bioactivity of Cereal
arabinoxylans in Relation to Their Sources
and Structure
Weili Li1, Zhengxiao Zhang2 , Jason Ashworth3, Andrew Fowell3 ,
Qiuyu Wang3 and Christopher Smith1
1University of Chester, Chester, UK
2Manchester Metropolitan University, UK
3Manchester Metropolitan University, UK
Arabinoxylans are major components of cereal cell
walls and they occur at higher content in the by-
products of milling, wheat brans, rich brans and rice hulls
of dietary fibre than in wheat flour and rice. Arabinoxylans
have been reported to have numerous health benefits in
recent studies. This presentation will report our recent
studies on effects of cereal arabinoxylan extracts with
various molecular weights and structures on their human
immunity modulation and anticancer activity in in vitro
testing.
The extraction yield and structure of AXs varied with
sources and extraction technologies. In this study, AXs
were extracted from wheat flour pentosan with and
without xylanase treatment. In in vitro testing, nitric oxide
(NO) secretion and inducible nitric oxide synthase (iNOS)
expression of human immune cells of U937 induced by
enzyme extracted AXs and water extracted AX were
compared. The results show that AXs treatments not only
enhanced NO production but also iNOS levels in U937
cells (P < 0.05) compared to untreated cells. The enzyme-
treated AXs with a higher proportion of low Mw AXs (1-
10KDa) and high A/X ratio (0.83) induced significantly
higher (P < 0.05) iNOS expression (132.2 ± 11.9 μg/
ml) than water-extracted AXs iNOS expression (104.3 ±
4.6μg/ml) and the increase in NO secretion corresponds
to iNOS concentration in cultured cells, which suggest a
pathway by which AXs modulate NO production in human
macrophage cells. In addition, It was also found that at a
concentration of 500µg/mL, enzyme-treated AXs caused a
more significant inhibition of proliferation of Gastric cancer
cells (p<0.05) and also more significantly reduced the
viability of Gastric cancer cells than water extracted AXs
following 24 and 48 hours treatment in in vitro (p<0.05).
Therefore, a potential application of AXs is potentially
used as a new method of treating gastric cancers.
Insights Nutr Metab 2017