nano-chemistry-2017-keynote

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Mater Sci Nanotechnol 2017 | Volume 1 Issue 2

allied

academies

Nanomaterials and Nanochemistry

November 29-30, 2017 | Atlanta, USA

International Conference on

redicting how proteins fold and adsorb onto surfaces is a

complex problem of strong relevance to the health and

environmental sectors. In this work, two nano-patterning

techniques, namely focused ion beam (FIB) milling and

atomic force microscopy (AFM) nanoindentation were

used to develop hydrogenated amorphous carbon (a-C:H)

model surfaces with similar nano-topography but different

local composition. On the un-patterned surfaces, bovine

plasma fibrinogen (BPF) resulted in a thicker and rougher

adsorbed film than bovine serum albumin (BSA), although

FTIR analysis indicated that, the secondary structure of the

proteins changed similarly, with an increase of the β-sheet

component (+27% and +34% for BSA and BPF, respectively).

AFM analysis on the FIB-patterned surfaces indicates that

patterning can modify specific protein adsorption behaviors.

Moreover, the patterns were compared by imaging the AFM

tip/surface adhesive force for BSA adsorbed on either AFM

tips or patterned surfaces. The results show an electrostatic

interaction between the implanted Ga+ and BSA surface,

modifying the adsorption behavior and the adhesive force.

Modeling this interaction gave an estimate of the surface

charge per protein, a significantly lower value than in dilute

solution (-1.8e instead of -18e). This finding is indicative of

protein misfolding, as detected in the FTIR analysis.

Speaker Biography

Dr. Patrick Lemoine works at the Nano-Integrated Bioengineering center (NIBEC) of

the Ulster University (UK). He graduated in 1989 from ENSPG-Grenoble (France),

completed his Ph.D in 1992 in Trinity College Dublin (Ireland) and also worked at Turin’s

Polytechnic Institute (Italy). His research is on ultrathin film analysis, particularly using

AFM techniques. He has attracted funding from the Royal Society and the Leverhulme

Trust, takes part in both industrial and academic research projects and has been key

note speaker and invited speaker at international conferences. He is a contributor to

specialist books on carbon materials (CRC Press and Springer Verlag) and has over 61

publications in peer-reviewed journals (h-index=17). He was a Committee member of

the IOP in Ireland (2002-2004) and is a member of the UU Research Ethics Committee.

Patrick Lemoine

University of Ulster, UK

Protein adsorption on nano-patterned hydrogenated amorphous carbon model

surfaces