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Microbiology: Current Research | Volume 3
May 20-21, 2019 | Vienna, Austria
Medical Microbiology
4
th
International Conference on
The potenial effect of probiotic bacteria against resistant-carpabenem
Acinetobacter baumennii
Neveen Mohamed Saleh, Sheren M Ahraf
and
Mahmoud M Motawaa
National Organization for Drug Control and Research, Egypt
I
nfections caused by carbapenem resistance
Acinetobacter
baumannii
(crab) are continually a focus of significant
attention since the limitation of therapeutic options. Probiotic
bacteria always have an essential role in dairy products
and fermented food and promoted the positive health
image as the treatment of microbial infections. Here, we
evaluated the potential of antimicrobial agent produced by
probiotics for the protection against crab infection. Tracheal
aspirate specimen from 150 patients at Egyptian hospitals
were recognized as Ab by PCR detection of blaOXA-51.
Antimicrobial susceptibility was studied. Positive crab
isolates with blaOXA-24 and blaOXA-58
incidence were
undergoing for screening using probiotics that have been
isolated from dairy products & food supplement. Probiotic
have highest antagonistic activity was identified and its
bioactive compounds were purified & characterized by
studying physiochemical characters. Nearly ninety-six of the
cases were crab & 37.5% of cases harboring blaOXA-24 & only
one case has blaOXA-58.
In vitro
, significantly 80% (P<0.05) of
crab remarkably inhibited by four probiotics.
Bifidobacterium
bifidum
strain that showed the highest activity against crab
has been identified, with significant inhibition levels reaching
83.33% in the case of the supernatant and even 97% inhibition
of supernatant purified by column chromatography. Purified
BbV1 was heat stable with amino acid content as identified by
LC-MS/MS and belonged to bacteriocins-like compounds. Our
finding demonstrated that natural BbV1 provides a protection
against
Acinetobacter
infection
in vitro
.
In vivo
, further studies
were applied using immunological and histological studies for
application as nutritional and pharmaceutical use.
e
:
salehneveen@yahoo.comMicrobiol Curr Res, Volume 3
DOI: 10.4066/2591-8036-C1-006