materials-summit-separation-summit-2018-posters

Page 43

O c t o b e r 1 9 - 2 0 , 2 0 1 8 | T o k y o , J a p a n

Materials Summit 2018 & Separation Summit 2018

Materials Science and Nanotechnology

Volume 2

ADVANCED MATERIALS AND POLYMER SCIENCE

SEPARATION TECHNIQUES

International Conference on

International Conference and Expo on

Joint Event on

OF EXCELLENCE

IN INTERNATIONAL

MEETINGS

YEARS

Mater Sci Nanotechnol 2018, Volume 2

EXTRACTION, SEPARATION AND DETERMINATION OF TETRACYCLINE IN

POULTRY USING (HPLC-DAD)

Lobaina M Alrhia

Tishreen University, Syria

his research deals with determine the optimal conditions for extraction, separation and determination of tetracycline

compound in poultry using high performance liquid chromatography technique with diode array detector (HPLC-DAD). The

study shows a good recovery ratio for tetracycline compound 87.50% at the extraction by ultrasonic water bath with a percentage

relative standard deviation less than 1% during half an hour. The best recovery ratio for tetracycline compound in poultry 89.55%

was recorded using an extraction solvent of Mcllvaine-EDTA at pH: 2.6. On the other hand, cartridges solid phase extraction

oasis HLB (hydrophilic lipophilic balance copolymer) show a good response for the recovery of tetracycline compound 86.0%

from poultry samples at a concentration of 0.1ppm in comparison with other cartridges. The recovery ratio of tetracycline

compound doesn´t affected by increasing the volume of methanol used in elution more than 3.5ml. The study mentioned that

the hold time needed for the quantitative determination of tetracycline compound from six poultry samples was about three and

half hours, which considered as a good time for the application of this method in the control laboratories.