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Journal of Industrial and Environmental Chemistry

|

Volume 2

GREEN CHEMISTRY &

TECHNOLOGY

7

th

International Conference on

J u n e 1 8 - 2 0 , 2 0 1 8 | D u b l i n , I r e l a n d

Atef Mohamed Khedr Nassar et al., J Ind Environ Chem 2018, Volume 2 | DOI: 10.4066/2591-7331-C1-002

EFFECTIVENESS OF BOTANICAL

NANOPARTICLES AGAINST ROOT-

KNOT NEMATODE (

MELOIDOGYNE

INCOGNITA

)

Atef Mohamed Khedr Nassar, Moustafa A Abbassy, Mona A

Abdelrasoul

and

Belal M Soliman

Damanhour University, Egypt

W

orldwide, the root-knot nematode (

Meloidogyne

spp.) is an invasive

plant pathogen that coulddestroyup to64%of the yield.Management

strategies mainly depend on the use of synthetic nematicides that have

adverse effects on human and the environment. Therefore, current study

investigated the effectiveness of leaf extracts of

Conyza dioscoridis

and

Urtica urens

that were prepared as silver nanoparticles (Ag-NP) as

nematicidal alternatives. The characterization and size confirmation of

the Ag-NP were done by UV-Vis spectrophotometry and the scanning

electronmicroscopy (SEM). The phytochemical contents of crude extracts

and the nano formulations were analyzed using gas chromatography-

mass spectroscopy (GC-MS). Results revealed that silver nanoparticles

of

C. dioscoridis

and

Urtica urens

extracts had great nematicidal activity

against the 2

nd

stage juvenile (J2) and eggs of

Meloidogyne incognita

.

Also, the Ag-NP showed similar nematicidal effect to the reference

nematicides. The GC-MS analysis revealed the increase of certain

metabolites due to the formulation of the Ag-NPs. Aromadendrene,

1-hydroxy-1,7-dimethyl-4-isopropyl-2,7-cyclodecadiene, 6-epi-shyobunol,

4-hexylacetophenone, β-isocomene, caryophyllene, β- and α-selinene,

α-cadinol, berkheyaradulen (

It’s a sesquiterpene hydrocarbon that is

detected in many plant species; Chrysanthemum boreale, chamomile oil,

and many others,

) and bis-(2-ethylhexyl)phthalate were increased more

than 2.5-folds in the Ag-NP compared to the extract. Therefore, the green

synthesis of metal nanoparticles might be a safe, effective and affordable

nematicide alternatives.

Atef Mohamed Khedr Nassar finished his BSc.

and MSc. degrees at Alexandria University,

Egypt and the PhD. degree at McGill Universi-

ty, Canada. He is working as an associate pro-

fessor of Pesticide Chemistry and Toxicology,

at Department of Plant Protection, Faculty of

Agriculture, Damanhour University, Egypt. He is

involved in studying the adverse effects of pes-

ticides to humans and non-target organisms.

In addition, he is trying to find new alternatives

to pesticides including nano-formulations of

pesticides and natural products to reduce the

amount of pesticides applied into the environ-

ment. He published many research articles in

peer-reviewed and highly impacted scientific

journals in the field of pest management and

pesticides residue analysis and toxicology.

Atef.nassar@dmu.edu.eg

BIOGRAPHY