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Ethnopharmacology 2019
& Physiotherapy Congress 2019
Asian Journal of Biomedical and Pharmaceutical Sciences
ISSN: 2249-622X | Volume 9
Page 31
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
alliedacademies.comYEARS
March 27-28, 2019 | Amsterdam, Netherlands
&
PHARMACOLOGY AND ETHNOPHARMACOLOGY
7
th
International Conference and Exhibition on
5
th
GLOBAL PHYSIOTHERAPY, PHYSICAL
REHABILITATION AND SPORTS MEDICINE
Joint Event on
EXTRACTS FROM ANTARCTIC YEASTS AND HEMP PLANT INHIBIT THE
GROWTH OF HUMAN LYMPHOID MALIGNANT CELLS
Spiro Konstantinov
1
, Antonios Trochopoulos
1
, Ervin Ivanov
1
, Snezhana Rusinova-Vide-
va
2,3
, Dilyana Hristova
1
, Maya M Zaharieva
2,3
, H Najdenski
2,3
and
Margarita Genova
4
1
Medical University of Sofia, Bulgaria
2
Bulgarian Academy of Sciences, Bulgaria
3
University of Plovdiv Paisii Hilendarski, Bulgaria
4
National Hematological Hospital, Bulgaria
Background:
Psychrophilic microorganisms successfully colonized deep seas, high mountains and Polar areas.
Antarctica offers permanent low temperatures, strong winds, short summer and intensive sun irradiation. To
survive under Antarctic conditions microorganisms, need to possess adaptable metabolism thus producing
bioactive components with attractive pharmacological properties.
Cannabidiol
is a component in hemp. It has
remarkable pharmacological activities such as tumor growth inhibition, pain perception modulation, and an-
ticonvulsive, antipsychotic and antiemetic properties as well. It lacks any psychotropic activity and common
toxicity and therefore is present in food supplements.
Methodology:
Amethanol extract from bioreactor grown yeast was prepared. The cytotoxic efficacy was mea-
sured using the MTT-assay. Induction of apoptosis was ascertained by nuclear changes, DNA fragmentation,
up- and down-regulation of pro- and anti-apoptotic proteins, PARP cleavage and fragmentation, as well as by
caspase activation. Inhibition of NF-κB was estimated by specific ELISA.
Results:
Concentration response curves showed IC50 values between 55 and 326 µg/ml for the Antarctic yeast
extract and bellow 30 µM for
Cannabidiol
. An up-regulation of pro-apoptotic signaling molecules such as Bad,
Bax, caspase 3, cytochrome c etc., as well as down-regulation of anti-apoptotic proteins such as Bcl-2, HSP-70,
clusterin etc. were detected. Nuclear fragmentation and cell cycle changes were demonstrated.
Cannabidiol
was found to reduce the activity of the NF-κB transcription factor to a comparable with that of curcumin extent.
Conclusions:
Cannabidiol
and the yeast extract have antineoplastic activity, which is comparable with that of
curcumin. Since both natural products are usually well tolerated and do not produce any toxic effects, there
is considerable merit in the development of Antarctic yeast and hemp plant extracts as potential therapy for
lymphoid neoplasms.
Spiro Konstantinov et al., Asian J Biomed Pharmaceut Sci 2019, Volume 9 | DOI: 10.4066/2249-622X-C1-017