14 / 15
Page 37
allied
academies
September 10-11, 2018 | Paris, France
&
Joint Event
Otolaryngology: ENT Surgery
6
th
International Conference on
Cell & Stem Cell Research
World Congress and Expo on
Journal of Otolaryngology Online Journal | Volume 8
Intestinal stem cell differentiation after massive small bowel resection is regulated by Notch signaling
in a rat model
Igor Sukhotnik
University of Michigan Medical School, USA
Objective:
Notch signaling promotes differentiation to
the absorptive cell lineage rather than to the secretory
cell lineage. The objective of this study was to determine
the role of Notch signaling in intestinal stem cell
differentiation in a rat model of short bowel syndrome (SBS).
Methods:
Male Sprague-Dawley rats were randomly assigned
to one of two experimental groups of 8 rats each: Sham rats
underwent bowel transection and re-anastomosis, SBS- rats
underwent 75% mall bowel resection. Rats were sacrificed on
day 14. Illumina’s Digital Gene Expression (DGE) analysis as used
to determine Notch signaling gene expression profiling. Notch-
related gene and protein expression were determined using
Real Time PCR, Western blotting and immunohistochemistry.
Results:
From 7 investigated Notch-related (by DGE analysis)
genes 6 genes were up-regulated in SBS vs control animals
with a relative change in gene expression level of 20%
or more. A significant up-regulation of Notch signaling
related genes in resected animals was accompanied by a
significant increase in Notch-1 protein levels (Western Blot)
and a significant increase in NOTCH-1 and Hes -1 (target
gene) positive cells (immunohistochemistry) compared
to sham animals. Evaluation of cell differentiation has
shown a strong increase in total number of absorptive
cells (unchanged secretory cells) compared to control rats.
Conclusions:
Twoweeksafterbowel resection inrats, stimulated
Notch signaling directs crypt cells population toward absorptive
progenitors.
e:
igor-dr@internet-zahav.netENT and Stem Cell 2018, Volume 8
DOI: 10.4066/2250-0359-C1-003