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N o v e m b e r 1 4 - 1 5 , 2 0 1 8 | R o m e , I t a l y
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Euro Diabetes Congress 2018, Global Vaccines Congress 2018
& Food Science Conference 2018 Archives of General Internal Medicine | ISSN: 2591-7951| Volume 2
&
&
NUTRITION, FOOD SCIENCE & TECHNOLOGY
International Conference on
DIABETES, ENDOCRINOLOGY & NURSING MANAGEMENT
World Congress on
VACCINES & VACCINATION
3rd Global Congress on
Keerthi Thalakattil Raghavan et al., Arch Gen Intern Med 2018, Volume 2 | DOI: 10.4066/2591-7951-C6-017
IMMUNITY & GUT MICROBIOME:
PROBIOTIC & PREBIOTIC AS COLONIC
BIOFERTILIZERS
Keerthi Thalakattil Raghavan, Honey Chandran C, Sreelakshmi
Kumar, JeenaSusan and Rakhi Narayanan
Mahatma Gandhi University, India
The GI tract is described as the body’s largest immune organ and the in-
testinal microbiota has a vital role in the body’s defense system. Further
the synergy of the probiotic and prebiotic components provides a stable and
relatively uniform gut microbiome. In the present study the possibility for
development of nutraceuticals using potent probiotic LAB strains isolated &
characterized from different natural sources & its enrichment by the addition
of suitable prebiotics such as honey, lnulin & accacia gum was investigated.
Honey is a natural source of prebiotic fructose oligosaccaride, Acacia gum is
also a natural source of prebiotic rich in arabinose,rhamnose and galactose
(PRAG). Inulin is a dietary fiber known as fructan. To evaluate the effect of
different prebiotics on probiotic, fermented milk samples were prepared by
adding honey, acacia gum and lnulin. Bacterial count, acidity and nutritional
aspects like protein, fat, and sugar content were determined. All the synbiot-
ic combinations exhibited higher functionability compared to probiotic only.
Synbiotic fermented samples, Honey exhibited the highest viability & higher
protein content but Acacia gum reduced the sugar and fat content which are
advantageous to diabetics & hypercholerstemic conditions. Both Honey &
Acacia gum exhibited antimicrobial activity towards selected common patho-
gens. Invivo study was conducted with selected probiotic & prebiotic Aca-
cia Gum & combination in experimental balb/c Mice during 3 weeks of oral
treatment. Results obtained for colonization, persistence and histopatholo-
gy study indicated significant level of colonization and no sign of infection.
Highest colonization was observed in synbiotic treated group suggesting the
role of prebiotic in enhanced growth of probiotic. Unlike with probiotic, Acacia
gum treatment suggested an anti-obesity role, which substantiates the result
of in vitro study. Levels of serum triglyceride and total cholesterol were within
control for the synbiotic treated group. The prebiotic treated group recorded
highest levels of Phosphorus and Calcium ions in the serum, indicating im-
pact of ion absorption ability. Humoral immunity in probiotic alone treated
group was marked by increased Phosphorus and Calcium ions in the serum,
indicating impact of ion absorption ability. Humoral immunity in probiotic
alone treated group was marked by increased level of serum IgA, IgM. Synbi-
otic combination exhibited phagocytic activity 3 times higher than individual
effect and probiotic could equally activate both T cell & B cell in response
to mitogens concanavalin A & lipopolysaccharide. It is a remarkable obser-
vation that a probiotic strain could induce both T cell & B cell. Probiotic was
Keerthi Thalakattil Raghavan has awarded her PhD from
Cochin University of Science and Technology, Cochin,
Kerala, India. Currently she is professor in School of Bio-
sciences, Mahatma Gandhi University, Kerala, India. Her
areas of interests are probiotics, prebiotic, microbiome
and bio prospecting of marine microbe. She is member
of number of academic and administrative bodies in her
university and other universities. She has Membership in
Scientific Societies such as International Probiotics As-
sociation (IPA), Zurich, Switzerland; Asian Federation of
Biotechnology (AFOB), Korea; Indian Dairy Association
(IDA), New Delhi.
BIOGRAPHY
able to stimulate TNF α production. Acacia gum
down regulated the production, suggesting that
it can be recommended as TNF α inhibitor/reg-
ulator. Liver cells have antioxidant property and
synbiotic group rendered more activity. Probiotic
& prebiotics have different mechanisms to pre-
vent colon cancer. Prominent reduction was ob-
served in the level of faecal colon cancer marker
enzymes β-glycosidase & β-glucuronidase in
treated group but higher reduction was noticed
in synbiotic combination. These results suggest
that synergy of suitable probiotic and prebiotic
can act as colonic biofertilizers.