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N o v e m b e r 1 4 - 1 5 , 2 0 1 8 | R o m e , I t a l y
Joint Event on
OF EXCELLENCE
IN INTERNATIONAL
MEETINGS
YEARS
Euro Diabetes Congress 2018, Global Vaccines Congress 2018
& Food Science Conference 2018 Archives of General Internal Medicine | ISSN: 2591-7951| Volume 2
&
&
NUTRITION, FOOD SCIENCE & TECHNOLOGY
International Conference on
DIABETES, ENDOCRINOLOGY & NURSING MANAGEMENT
World Congress on
VACCINES & VACCINATION
3rd Global Congress on
Oksana Ryabinina Debrah et al., Arch Gen Intern Med 2018, Volume 2 | DOI: 10.4066/2591-7951-C6-017
RELATIONSHIP BETWEEN CERVICAL
EPITHELIAL CELL ABNORMALITIES AND
CO-INFECTION WITH HERPES SIMPLEX
VIRUS AND HUMAN PAPILLOMAVIRUS
AMONG UNSCREENED WOMEN IN GHANA
Oksana Ryabinina Debrah1,2 , Francis Agyemang-Yeboah1,
Emmanuel Timmy-Donkoh1,5, Richard Harry Asmah3, Mustapha
Mohammed Seini1,2 and Ellis Owusu-Dabo4
1Kwame Nkrumah University of Science and Technology, Ghana
2Greater Accra Regional Hospital, Ghana
3University of Ghana, Ghana
4KNUST, Ghana
5Kwame Nkrumah University of Science and Technology, Ghana
Herpes simplex virus type 1 and type 2 are responsible for recurrent
oro-genital ulcers, complicated childbirths and significant morbidity
globally. HPV also infect the female genital region and the most prominent
risk factor of cervical cancer. HSV may act in conjunction with HPV in the
development of cervical epithelial lesions. Most vulnerable women in Ghana
may be oblivion of fact that they live with HPV/HSV co-infection. In order to
investigate the impact of HPV and HSV co-infection in the etiology of cervi-
cal epithelial cell abnormalities, women attending the Cervicare Centers in
Ghana were invited to participate in cross-sectional study. Cytological speci-
mens were obtained from all subjects for Pap smear test. ELISA was used to
detect type-specific IgG against HSV-1 and HSV-2 antibodies. Genomic DNA
from cervical swabs was extracted using QIAamp Mini kit. HPV-DNA detec-
tion were carried out by nested multiplex PCR as s described by Sotlar et
al., (2004). The SPSS version 22 was used for statistical analysis. Statistical
significance was accepted for p<0.05. Our result show that among women
with abnormal cytology the prevalence of HPV infection of any type, HSV-
1 and HSV-2 were 55.6%, 90.0% and 70.0% respectively. Co-infection HPV/
HSV-1 and HPV/HSV-2 was 44.4% in both cases with cervical epithelial cell
abnormalities. There was a low awareness of the possible interaction of HSV
and HPV with the development of cervical cancer among study participants.
However, the study did not observe any significant association between co-in-
fection of two viruses and cervical epithelial cell abnormalities (p=0.343 for
HSV-1/HPV and p=0.274 for HSV-2/HPV, respectively). We do recommend
early case detection for all women with HSV and vaccination against HPV to
decrease the risk of HPV acquisition and cervical cancer development.
Oksana Ryabinina Debrah has MSc in clinical biochem-
istry from Donetsk National University, Ukraine), she has
done her MPh in Medical Biochemistry from University
of Ghana, Legon and PhD in chemical pathology from
Kwame Nkrumah University of Science and Technolo-
gy, Ghana. She has a 15 years of experience as a med-
ical laboratory scientist at Ridge Regional Hospital, Ac-
cra-Ghana, where she worked as the head of chemical
pathology unit at the laboratory department. She is cur-
rently the deputy chief medical laboratory scientist at the
Institutional Care (Clinical Care) Division of the Ghana
Health Service, as well as a lecturer at Accra Technical
University- Ghana. Her research interests are in the de-
velopment of novel biochemical markers which can be
used in screening programs for disease prediction, and
in the epidemiology of HSV and its synergism with other
sexually transmitted viruses, vaccine development. She
also has ongoing research in the area of bacterial and/
or viral epidemiology and transmission within the context
of hospital infection control and prevention. Her publica-
tions in area of biochemical markers and infectious dis-
eases.
BIOGRAPHY